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Invitrogen

STAT3 Monoclonal Antibody (9D8)

Advanced Verification
13 Published Figures
34 References
View all (104) STAT3 antibodies

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STAT3 Monoclonal Antibody (9D8)

Product Details

MA1-13042

Applications
Tested Dilution
Publications

Western Blot (WB)

1:5000-1:10,000
View 25 publications 25 publications

Immunohistochemistry (IHC)

-
View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,600
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100
View 3 publications 3 publications

Immunoprecipitation (IP)

2 µg
-

ChIP assay (ChIP)

1-3 µL
View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

9D8

Immunogen

Residues 655-770 from human recombinant protein expressed in bacteria
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10985240

Product Specific Information

MA1-13042 detects STAT3 from human, mouse, monkey and rat samples.



MA1-13042 has been successfully used in Western blot, ChIP, immunofluorescence, immunohistochemistry (paraffin), and immunoprecipitation procedures. By Western blot, this antibody detects a 88 kDa protein

corresponding to STAT3. Positive control: HepG2 cells.



The MA1-13042 antigen is a recombinant protein corresponding to residues 655-770 of human STAT3.



Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

Target Information

STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: acute phase response factor; Acute-phase response factor; DNA-binding protein APRF; FLJ20882; MGC16063; Signal transducer and activator of transcription 3; STAT; Stat3; unnamed protein product

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Gene Aliases: 1110034C02Rik; ADMIO; ADMIO1; APRF; AW109958; HIES; STAT3

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UniProt ID: (Human) P40763, (Rat) P52631, (Mouse) P42227

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Entrez Gene ID: (Human) 6774, (Rat) 25125, (Mouse) 20848

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding RNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding receptor binding protein binding protein kinase binding protein phosphatase binding chromatin DNA binding CCR5 chemokine receptor binding glucocorticoid receptor binding signaling adaptor activity identical protein binding protein homodimerization activity sequence-specific DNA binding protein dimerization activity RNA polymerase II sequence-specific DNA binding transcription factor binding primary miRNA binding lncRNA binding DNA-binding transcription factor binding protein sequestering activity RNA sequestering activity RNA polymerase II repressing transcription factor binding signal transducer activity transcription factor binding transcription regulatory region DNA binding DNA-binding transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter temperature homeostasis response to hypoxia eye photoreceptor cell differentiation regulation of cytokine production response to ischemia regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein import into nucleus defense response acute-phase response inflammatory response immune response signal transduction transforming growth factor beta receptor signaling pathway I-kappaB kinase/NF-kappaB signaling JAK-STAT cascade nervous system development cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation gene expression negative regulation of autophagy positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression negative regulation of hydrogen peroxide biosynthetic process phosphorylation cytokine-mediated signaling pathway sexual reproduction signal transduction involved in regulation of gene expression cell differentiation positive regulation of cell migration intracellular receptor signaling pathway response to estradiol positive regulation of interleukin-1 beta production positive regulation of interleukin-10 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production cellular response to hormone stimulus leptin-mediated signaling pathway response to cytokine somatic stem cell population maintenance interleukin-15-mediated signaling pathway interleukin-2-mediated signaling pathway interleukin-9-mediated signaling pathway interleukin-11-mediated signaling pathway interleukin-23-mediated signaling pathway regulation of multicellular organism growth regulation of cell proliferation glucose homeostasis eating behavior mRNA transcription from RNA polymerase II promoter positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling response to peptide hormone protein kinase B signaling cellular response to leptin stimulus response to leptin response to ethanol positive regulation of erythrocyte differentiation positive regulation of Notch signaling pathway positive regulation of angiogenesis negative regulation of glycolytic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of mitochondrial membrane permeability astrocyte differentiation negative regulation of inflammatory response positive regulation of inflammatory response positive regulation of phagocytosis modulation of synaptic transmission positive regulation of multicellular organismal process regulation of cell cycle radial glial cell differentiation retinal rod cell differentiation regulation of feeding behavior growth hormone receptor signaling pathway JAK-STAT cascade involved in growth hormone signaling pathway interleukin-6-mediated signaling pathway cellular response to cytokine stimulus T-helper 17 type immune response T-helper 17 cell lineage commitment positive regulation of extracellular matrix disassembly inflammatory response to wounding energy homeostasis cellular response to interleukin-17 receptor signaling pathway via STAT postsynapse to nucleus signaling pathway negative regulation of inflammatory response to wounding interleukin-10-mediated signaling pathway negative regulation of cytokine production involved in inflammatory response positive regulation of cytokine production involved in inflammatory response regulation of cellular response to hypoxia positive regulation of growth factor dependent skeletal muscle satellite cell proliferation positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of vascular endothelial cell proliferation negative regulation of 3'-UTR-mediated mRNA stabilization negative regulation of primary miRNA processing negative regulation of stem cell differentiation positive regulation of ATP biosynthetic process negative regulation of neuron migration aging response to organic substance response to organic cyclic compound stem cell population maintenance response to drug negative regulation of cell death negative regulation of neuron death transcription, DNA-templated negative regulation of apoptotic process
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