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Invitrogen
RAD21 Monoclonal Antibody (CM110-2C10)
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FIGURE: 1 / 3
RAD21 Antibody (MA1-16585) in WB
Western blot was performed using RAD21 Monoclonal Antibody (CM110-2C10) (Product # MA1-16585) and a band at ~90 kDa corresponding to RAD21 was decreased upon etoposide treatment in HeLa. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), HeLa treated with 50µM Etoposide for 48 hrs (Lane 2) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The b... View More
Product Details
MA1-16585
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
CM110-2C10
Immunogen
An N-terminal fusion protein of the C-terminal fragment of Scc1 subunit human cohesin (amino acids 544-631 of hRad21).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.1% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In Western blot, two bands are seen: the full-length Rad21 is seen around 130 kDa, while the cleaved Rad21 is seen around 70 kDa.
Suggested positive control: antigen standard for RAD21 (transient overexpression lysate), Hela whole cell extract.
Target Information
This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Alternative splicing results in multiple transcripts variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Double-strand-break repair protein rad21 homolog; FLJ25655; FLJ40596; h NXP-1; hHR21; HR21spA; KIAA0078; KIAA0078RAD21; kleisin; mHR21; Nuclear matrix protein 1; NXP-1; Pokeweed agglutinin-binding protein 29; protein involved in DNA double-strand break repair; PW29; RAD21 homolog; SCC1 homolog; sister chromatid cohesion 1; unnamed protein product
Gene Aliases: CDLS4; hHR21; HR21; HRAD21; KIAA0078; MCD1; MGS; mKIAA0078; NXP1; RAD21; SCC1
UniProt ID: (Human) O60216, (Mouse) Q61550
Entrez Gene ID: (Human) 5885, (Mouse) 19357
response to hypoxia
DNA repair
double-strand break repair
DNA recombination
regulation of transcription from RNA polymerase II promoter
apoptotic process
cellular response to DNA damage stimulus
chromosome segregation
sister chromatid cohesion
reciprocal meiotic recombination
positive regulation of gene expression
negative regulation of gene expression
negative regulation of G2/M transition of mitotic cell cycle
negative regulation of interleukin-1 beta production
negative regulation of tumor necrosis factor production
positive regulation of interleukin-10 production
establishment of mitotic sister chromatid cohesion
establishment of meiotic sister chromatid cohesion
negative regulation of glial cell apoptotic process
negative regulation of neuron apoptotic process
negative regulation of mitotic metaphase/anaphase transition
positive regulation of sister chromatid cohesion
cell division
protein localization to chromatin
chromatin looping
replication-born double-strand break repair via sister chromatid exchange
transcription from RNA polymerase II promoter
cell cycle
mitotic nuclear division
positive regulation of transcription from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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