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Invitrogen
HNF4A Monoclonal Antibody (K9218)
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FIGURE: 1 / 20
HNF4A Antibody (MA1-199) in ICC/IF
Immunofluorescence analysis of HNF4A was performed using 70% confluent log phase HepG2 and HeLa cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Anti-HNF4A Monoclonal Antibody (Product # MA1-199) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjug... View More
Product Details
MA1-199
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
K9218
Immunogen
Baculovirus-expressed recombinant human HNF4 alpha (aa 3-49)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
physiological saline
Contains
0.1% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-199 detects HNF4a in human, mouse and rat samples.
Target Information
Hepatocyte nuclear factor 4 alpha (HNF4 alpha) is an orphan nuclear receptor. HNF4a is expressed in the liver, kidney, intestine, and pancreas. Mutation of HNF4a in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4a gene is alternatively spliced and may generate up to nine different isoforms, HNF4a1 through HNF4a9.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha transcription factor 4; FLJ39654; hepatic nuclear factor 4 alpha; hepatic nuclear factor 4, alpha; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; HNF4 alpha; HNF4-alpha; HNF4A wild type; HNF4a7; HNF4a8; HNF4a9; HNF4alpha10; HNF4alpha10/11/12; HNF4alpha11; HNF4alpha12; Nuclear receptor 2A1; Nuclear receptor subfamily 2 group A member 1; TCF-14; transcription factor; Transcription factor 14; Transcription factor HNF-4
Gene Aliases: FRTS4; HNF-4; HNF4; HNF4A; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF-14; TCF14
UniProt ID: (Human) P41235, (Mouse) P49698, (Rat) P22449
Entrez Gene ID: (Human) 3172, (Mouse) 15378, (Rat) 25735
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
receptor binding
fatty acid binding
protein binding
zinc ion binding
protein homodimerization activity
sequence-specific DNA binding
metal ion binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
fatty-acyl-CoA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
steroid hormone receptor activity
transcription factor binding
palmitoyl-CoA hydrolase activity
transcription regulatory region DNA binding
repressing transcription factor binding
C4 zinc finger nuclear receptor
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
lipid metabolic process
xenobiotic metabolic process
sex differentiation
blood coagulation
negative regulation of cell proliferation
response to glucose
gene expression
regulation of gastrulation
regulation of lipid metabolic process
signal transduction involved in regulation of gene expression
cell differentiation
negative regulation of cell growth
intracellular receptor signaling pathway
glucose homeostasis
cholesterol homeostasis
regulation of circadian rhythm
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
regulation of insulin secretion
lipid homeostasis
phospholipid homeostasis
regulation of growth hormone receptor signaling pathway
triglyceride homeostasis
regulation of ornithine metabolic process
transcription, DNA-templated
ornithine metabolic process
acyl-CoA metabolic process
establishment of tissue polarity
regulation of microvillus assembly
negative regulation of protein import into nucleus, translocation
negative regulation of tyrosine phosphorylation of Stat5 protein
steroid hormone mediated signaling pathway
negative regulation of sequence-specific DNA binding transcription factor activity
cell-cell junction organization
positive regulation of gluconeogenesis
positive regulation of fatty acid biosynthetic process
negative regulation of mitotic cell cycle
SMAD protein signal transduction
negative regulation of activation of JAK2 kinase activity
positive regulation of cholesterol homeostasis
response to drug
response to cAMP
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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