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Invitrogen
NEFL Monoclonal Antibody (DA2)
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FIGURE: 1 / 14
NEFL Antibody (MA1-2010) in ICC/IF
Immunofluorescence analysis of Neurofilament, Light chain was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Neurofilament, Light chain (DA2) Mouse Monoclonal Antibody (Product # MA1-2010) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa ... View More
Product Details
MA1-2010
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig,
Rat
Published species
Chicken,
Human,
Mouse
Host/Isotype
Mouse
Class
Monoclonal
Type
Antibody
Clone
DA2
Immunogen
Enzymatically dephosphorylated porcine neurofilaments.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-2010 detects the neurofilament, light chain in human, rat, and mouse samples.
MA1-2010 has been successfully used in western blot, immunohistochemistry, and immunofluorescence. By western blot this antibody detects a ~70 kDa protein representing the neurofilament, light chain in mouse brain tissue lysate. In immunohistochemistry procedures MA1-2010 recognizes the neurofilament, light chain in mouse brain tissue. In immunofluorescence procedures MA1-2010 recognizes the neurofilament, light chain in rat cerebral cortices.
The MA1-2010 immunogen is enzymatically dephosphorylated porcine neurofilaments.
Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 68 kDa neurofilament protein; FLJ53642; intermediate filament; light molecular weight neurofilament protein; N-FL; NEF3; NEFL; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NF-M; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110; unnamed protein product
Gene Aliases: AI847934; CMT1F; CMT2E; CMTDIG; NEFL; NF-L; NF68; NFL; PPP1R110
UniProt ID: (Human) P07196, (Mouse) P08551, (Rat) P19527
Entrez Gene ID: (Human) 4747, (Pig) 100521224, (Mouse) 18039, (Rat) 83613
structural constituent of cytoskeleton
protein binding
tubulin binding
protein domain specific binding
protein binding, bridging
identical protein binding
phospholipase binding
macromolecular complex binding
structural constituent of postsynaptic intermediate filament cytoskeleton
protein C-terminus binding
structural molecule activity
protein heterodimerization activity
microtubule cytoskeleton organization
axonogenesis
anterograde axonal transport
retrograde axonal transport
response to toxic substance
peripheral nervous system axon regeneration
axonal transport of mitochondrion
spinal cord development
hippocampus development
cerebral cortex development
regulation of axon diameter
neurofilament bundle assembly
locomotion
response to peptide hormone
intermediate filament polymerization or depolymerization
intermediate filament organization
intermediate filament bundle assembly
neuron projection morphogenesis
positive regulation of axonogenesis
neuromuscular process controlling balance
protein polymerization
response to corticosterone
neurofilament cytoskeleton organization
regulation of synapse maturation
motor neuron apoptotic process
postsynaptic modulation of chemical synaptic transmission
postsynaptic intermediate filament cytoskeleton organization
response to sodium arsenite
response to acrylamide
negative regulation of motor neuron apoptotic process
axon development
negative regulation of neuron apoptotic process
response to organic substance
response to organonitrogen compound
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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