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Invitrogen
NEFM Monoclonal Antibody (3H11)
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FIGURE: 1 / 11
NEFM Antibody (MA1-2011) in ICC/IF
Immunofluorescence analysis of NF-M was performed using 70% confluent log phase HEK-293 and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. HEK-293 cells were labeled with NF-M Mouse Monoclonal Antibody (Product # MA1-2011) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (... View More
Product Details
MA1-2011
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Mouse
Class
Monoclonal
Type
Antibody
Clone
3H11
Immunogen
Synthetic peptide corresponding to a C-terminal fragment of rat neurofilament.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-2011 detects the neurofilament, medium chain in human and rat samples.
MA1-2011 has been successfully used in Western blot, immunofluorescent, and immunoprecipitation procedures. By Western blot this antibody detects a ~160 kDa protein representing the neurofilament, medium chain in HEK 293 cells, rat spinal cord, and human frontal cortex. In immunofluorecence procedures MA1-2011 recognizes the neurofilament, medium chain in human neurons.
The MA1-2011 immunogen is a synthetic peptide corresponding to a C-terminal fragment of rat neurofilament.
Target Information
Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 160 kDa neurofilament protein; NEFM; Neurofilament 3; neurofilament 3, medium; Neurofilament medium polypeptide; Neurofilament protein, middle polypeptide; Neurofilament triplet M protein; neurofilament triplet M protein; 160 KD neurofilament protein, NF-M; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); NF-M; NMC; unnamed protein product
Gene Aliases: NEF3; NEFM; NF-M; NFM
UniProt ID: (Human) P07197, (Rat) P12839
Entrez Gene ID: (Human) 4741, (Rat) 24588
neurofilament bundle assembly
microtubule cytoskeleton organization
axo-dendritic transport
spinal cord development
axon regeneration
regulation of axon diameter
cellular response to oxidative stress
intermediate filament cytoskeleton organization
intermediate filament polymerization or depolymerization
intermediate filament bundle assembly
neurofilament cytoskeleton organization
cellular response to estradiol stimulus
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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