Search
Invitrogen
NF-H Monoclonal Antibody (3G3)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-2012
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Fruit fly,
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Mouse
Class
Monoclonal
Type
Antibody
Clone
3G3
Immunogen
Synthetic peptide corresponding to 226 amino acids of the C-terminus of neurofilament, heavy chain.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-2012 detects the neurofilament, heavy chain in human and rat samples.
MA1-2012 has been successfully used in Western blot, immunohistochemistry and immunofluorecence procedures. By Western blot this antibody detects a ~200 kDa protein representing the neurofilament, heavy chain in rat brain microsome. In immunohistochemistry procedures MA1-2012 recognizes the neurofilament, heavy chain in rat hippocampal neurons.
The MA1-2012 immunogen is a synthetic peptide corresponding to 226 amino acids of the C-terminus of neurofilament, heavy chain.
Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 200 kDa neurofilament protein; heavy neurofilament polypeptide; heavy neurofilament subunit; high molecular weight neurofilament; hSTEp; NEF3; neurofilament; neurofilament triplet H protein; neurofilament, heavy polypeptide; neurofilament, heavy polypeptide 200kDa; NF 200 kD; NF-H; NHC; unnamed protein product
Gene Aliases: CMT2CC; NFH
UniProt ID: (Human) P12036
Entrez Gene ID: (Human) 4744, (Rat) 24587
axonogenesis
cell projection assembly
neurofilament bundle assembly
intermediate filament bundle assembly
axon development
postsynaptic modulation of chemical synaptic transmission
postsynaptic intermediate filament cytoskeleton organization
regulation of organelle transport along microtubule
microtubule cytoskeleton organization
ovarian follicle atresia
spinal cord development
hippocampus development
cerebral cortex development
axon regeneration
neuron projection development
cellular response to oxidative stress
response to cocaine
intermediate filament cytoskeleton organization
peripheral nervous system neuron axonogenesis
neurofilament cytoskeleton organization
cellular response to estradiol stimulus
cellular response to hydroperoxide
response to sodium arsenite
response to acrylamide
cellular response to leukemia inhibitory factor
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support