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Invitrogen
Bi-1 Monoclonal Antibody (20F430)
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FIGURE: 1 / 6
Bi-1 Antibody (MA1-41108) in IHC (P)
Immunohistochemistry analysis of Bi-1 in formalin-fixed, paraffin-embedded human adenocarcinoma of the rectum. Samples were incubated with Bi-1 monoclonal antibody (Product # MA1-41108) using a dilution of 5 µg/mL. Peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT fo... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-41108
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
20F430
Immunogen
A synthetic peptide made to the N-terminal portion of the human Bax inhibitor 1 protein (between amino acids 1-40).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS with 0.2% gelatin
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Epitope mapping indicates that the antibody recognizes an epitope in the N-terminal region of Bi-1.
Target Information
BI-1 (Bax Inhibitor 1) is in anti-apoptotic protein that has been linked to protection from apoptosis induced endoplasmic reticulum (ER) stress (reviewed in Bailley-Maitre et al, 2007). BI-1 contains several transmembrane domains, localizes to ER membranes, and has cytoprotective functions that are conserved in both animals and plants. BI-1 suppresses apoptosis induced by ectopic expression of the proapoptotic protein Bax as well as other types of stimuli. Cells from BI-1 knockout (KO) mice are hypersensitivite to apoptosis induced by ER stress-causing chemical agents (thapsigargin, tunicamycin, and brefeldin A) or by ischemia-reperfusion (IR) injury. Conversely, overexpression of BI-1 protects against apoptosis induced by ER stress and IR. The mechanism by which BI-1 protects cells from ER-stress induced apoptosis remains to be fully elucidated, itis thought to involve regulation of Ca2+ handling by the ER.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Bax inhibitor-1; BI-1; liver regeneration-related protein LRRG018; liver regeneration-related protein LRRG163; TEGT; testis enhanced gene transcript; testis-enhanced gene transcript protein; testis-enhanced gene transcript protein; integral membrane protein; transmembrane BAX inhibitor motif-containing protein 6; unnamed protein product
Gene Aliases: 5031406P05Rik; Ab1-011; Ac1-149; BAXI1; BI-1; Bi1; Cc1-27; TEGT
UniProt ID: (Human) P55061, (Mouse) Q9D2C7, (Rat) P55062
Entrez Gene ID: (Human) 7009, (Mouse) 110213, (Rat) 24822
negative regulation of transcription from RNA polymerase II promoter
negative regulation of immunoglobulin production
negative regulation of calcium ion transport into cytosol
endoplasmic reticulum calcium ion homeostasis
negative regulation of RNA splicing
cellular response to unfolded protein
response to endoplasmic reticulum stress
IRE1-mediated unfolded protein response
negative regulation of apoptotic process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
calcium ion transmembrane transport
response to L-glutamate
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
negative regulation of IRE1-mediated unfolded protein response
negative regulation of apoptotic signaling pathway
spermatogenesis
lung development
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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