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Invitrogen
CD142 Monoclonal Antibody (5264)
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FIGURE: 1 / 2
CD142 Antibody (MA1-43029) in ICC/IF
Immunofluorescence analysis of CD142 was performed using 70% confluent log phase MDA-MB-231 and MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD142 Monoclonal Antibody (5264) (Product # MA1-43029) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-43029
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
5264
Immunogen
Human Tissue Factor.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
4.7 mg/mL
Amount
100 µg
Purification
SDS-PAGE
Storage buffer
50% water with 50% glycerol
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-43029 detects Tissue Factor in human samples.
MA1-43029 has successfully been used in Western blot and ELISA applications.
MA1-43029 was derived from human tissue factor.
Target Information
This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: coagulation factor; coagulation factor III (thromboplastin, tissue factor); F3; tissue factor precursor; tissue factor versions 1 and 2 precursor; unnamed protein product
Gene Aliases: CD142; TF; TFA
UniProt ID: (Human) P13726
Entrez Gene ID: (Human) 2152
positive regulation of endothelial cell proliferation
activation of plasma proteins involved in acute inflammatory response
activation of blood coagulation via clotting cascade
proteolysis
response to stress
blood coagulation
blood coagulation, intrinsic pathway
blood coagulation, extrinsic pathway
response to wounding
positive regulation of gene expression
positive regulation of platelet-derived growth factor receptor signaling pathway
protein processing
cytokine-mediated signaling pathway
positive regulation of cell migration
positive regulation of TOR signaling
positive regulation of interleukin-8 production
positive regulation of angiogenesis
positive regulation of positive chemotaxis
blood coagulation, fibrin clot formation
positive regulation of endothelial cell apoptotic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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