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AHR Monoclonal Antibody (RPT1)
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FIGURE: 1 / 54
AHR Antibody (MA1-514) in ICC/IF
Immunofluorescence analysis of AHR was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with AHR Monoclonal Antibody (RPT1) (Product # MA1-514) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A28175... View More
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
.jpg?time=20240507?time=20240507 "AHR Antibody (MA1-514) in WB")
.jpg?time=20240507?time=20240507 "AHR Antibody (MA1-514) in WB")
Product Details
MA1-514
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Non-human primate,
Pig
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
RPT1
Immunogen
Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of human AhR.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-514 detects the aryl hydrocarbon receptor (AhR) from human, mouse, and rat cells.
MA1-514 has been successfully used in Western blotting, immunoprecipitation, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects an ~96 kDa protein representing AhR, with some minor non-specific bands observed in certain samples.
MA1-514 immunogen is residues 12-31 from the human AhR protein with amino acids 18-21 being omitted.
Target Information
AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ah receptor; AH-receptor; AHR; aromatic hydrocarbon receptor; class E basic helix-loop-helix protein 76; dioxin receptor; unnamed protein product
Gene Aliases: Ah; Ahh; Ahre; bHLHe76; FVH3; In; RP85
UniProt ID: (Human) P35869, (Mouse) P30561
Entrez Gene ID: (Human) 196, (Mouse) 11622, (Rat) 25690
transcription regulatory region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
TFIID-class transcription factor binding
transcription coactivator binding
DNA binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
TBP-class protein binding
protein homodimerization activity
protein heterodimerization activity
protein dimerization activity
Hsp90 protein binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
E-box binding
sequence-specific double-stranded DNA binding
toxic substance binding
aryl hydrocarbon receptor binding
identical protein binding
sequence-specific DNA binding
chaperone binding
arachidonic acid omega-hydroxylase activity
promoter-specific chromatin binding
blood vessel development
regulation of adaptive immune response
negative regulation of T cell mediated immune response to tumor cell
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
xenobiotic metabolic process
apoptotic process
response to xenobiotic stimulus
response to toxic substance
regulation of gene expression
intracellular receptor signaling pathway
regulation of B cell proliferation
circadian regulation of gene expression
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of inflammatory response
regulation of multicellular organismal process
cellular response to molecule of bacterial origin
cellular response to cAMP
cellular response to forskolin
cellular response to 2,3,7,8-tetrachlorodibenzodioxine
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis
ovarian follicle development
patterning of blood vessels
conditioned taste aversion
B cell homeostasis
regulation of cytokine production
liver development
B-1 B cell homeostasis
vasculature development
blood vessel remodeling
lymphocyte homeostasis
immune system process
negative regulation of systemic arterial blood pressure
cardiac left ventricle morphogenesis
circumferential growth involved in left ventricle morphogenesis
transcription from RNA polymerase II promoter
blood circulation
B cell differentiation
intracellular estrogen receptor signaling pathway
prostate gland development
negative regulation of osteoblast proliferation
protein localization to nucleus
post-embryonic hemopoiesis
tube development
positive regulation of growth rate
camera-type eye development
T cell homeostasis
positive regulation of apoptotic process
negative regulation of osteoblast differentiation
positive regulation of cell size
positive regulation of mating-type specific transcription, DNA-templated
positive regulation of RNA polymerase II transcriptional preinitiation complex assembly
negative regulation of vasoconstriction
animal organ development
blood vessel morphogenesis
spleen development
reproductive structure development
gland development
tissue remodeling
negative regulation of epithelial cell proliferation
contact inhibition
regulation of heart growth
developmental growth involved in morphogenesis
intestinal epithelial structure maintenance
mammary duct terminal end bud growth
kidney morphogenesis
glomerulus morphogenesis
cellular response to toxic substance
omega-hydroxylase P450 pathway
negative regulation of calcium ion transmembrane transport
reactive oxygen species biosynthetic process
regulation of eating behavior
cellular response to 3-methylcholanthrene
negative regulation of DNA biosynthetic process
female pregnancy
circadian rhythm
response to estradiol
nitric oxide metabolic process
response to arsenic-containing substance
regulation of cell cycle
cellular response to glucocorticoid stimulus
cellular response to dexamethasone stimulus
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