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Invitrogen
Cytokeratin Pan Type I/II Antibody Cocktail
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FIGURE: 1 / 10
Cytokeratin Pan Type I/II Antibody (MA1-82041) in ICC/IF
Immunofluorescence analysis of Cytokeratin Pan Type I/II was performed using 70% confluent log phase A-431 cells. The cells were fixed and permeabilized with ice-cold acetone at 4°C for 5 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin Pan Type I/II Antibody Cocktail (Product # MA1-82041, 1:100 dilution) in 0.1% BSA, incubated at 4 degree celsius overn... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-82041
Applications
Tested Dilution
Publications
Product Specifications
Published species
Chicken,
Horse,
Human,
Mouse,
Non-human primate,
Rat,
Sheep,
Zebrafish
Host/Isotype
Mouse
/ IgG1
Class
Cocktail
Type
Antibody
Clone
AE1/AE3
Immunogen
Human epidermal keratins.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Storage conditions
4°C or -20°C if preferred
Shipping conditions
Wet ice
RRID
Product Specific Information
Heat-mediated antigen retrieval is recommended for the staining of paraffin sections. A suggested positive control is human skin.
This antibody detects the acidic and basic (Type I and II) cytokeratins: Cytokeratin 1, Cytokeratin 2, Cytokeratin 3, Cytokeratin 4, Cytokeratin 5, Cytokeratin 6, Cytokeratin 7, Cytokeratin 8, Cytokeratin 10, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16 and Cytokeratin 19.
Mouse anti Human Cytokeratin (Pan) antibody, clone AE1/AE3 is a pan-cytokeratin reagent, being a cocktail of clones AE1 and AE3 which provides the broadest spectrum of reactivity to the 19 known human epidermal keratins.
Target Information
Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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