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Invitrogen
GLUT4 Monoclonal Antibody (1F8)
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FIGURE: 1 / 5
GLUT4 Antibody (MA1-83191) in WB
Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to adipocytes (Lane 2), HeLa (Lane 3), Hep G2 (Lane 4), NIH/3T3 (Lane 5), C2C12 (Lane 6), tissue extracts of Mouse heart (Lane 7), Mouse Skin (Lane 8) and Mouse Skeletal Muscle (Lane 9). The blot was probed with Anti-GLUT4 Monoclonal Antibody (1F8) (Product # MA1-83191, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-83191
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat
Published species
Human,
Rabbit
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1F8
Immunogen
Partially purified vesicles containing insulin-responsive glucose transporter 4
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Protein G
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody does not react with dog.
This product requires enzyme-mediated antigen retrieval prior to staining of paraffin sections. Proteinase K is recommended.
Mouse anti glucose transporter 4 antibody, clone 1F8 originally raised against rat intracellular low density microsomes (James et al.
Target Information
This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: glucose transporter 2; glucose transporter 4; glucose transporter 4 insulin-responsive; Glucose transporter 4, insuline-responsive; glucose transporter glut4; glucose transporter type 4; glucose transporter type 4, insulin-responsive; GLUT-4; insulin responsive glucose transporter; insulin-responsive glucose transporter; insulin-responsive glucose transporter type 4; MGC93607; solute carrier family 2 (facilitated glucose transporter), member 4; solute carrier family 2 , member 4; unnamed protein product
Gene Aliases: Glut-4; GLUT4; GT2; twgy
UniProt ID: (Human) P14672, (Mouse) P14142, (Rat) P19357
Entrez Gene ID: (Rabbit) 100037710, (Human) 6517, (Pig) 396754, (Mouse) 20528, (Rat) 25139
learning or memory
short-term memory
long-term memory
amylopectin biosynthetic process
positive regulation of brain-derived neurotrophic factor receptor signaling pathway
cellular response to insulin stimulus
glucose homeostasis
glucose import in response to insulin stimulus
response to ethanol
brown fat cell differentiation
transmembrane transport
dehydroascorbic acid transport
cellular response to tumor necrosis factor
cellular response to hypoxia
cellular response to osmotic stress
regulation of synaptic vesicle budding from presynaptic endocytic zone membrane
glucose import across plasma membrane
glucose transmembrane transport
carbohydrate metabolic process
white fat cell proliferation
transport across blood-brain barrier
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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