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Invitrogen
DYKDDDDK Tag Monoclonal Antibody (FG4R)
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FIGURE: 1 / 66
DYKDDDDK Tag Antibody (MA1-91878) in ICC/IF
Immunofluorescent analysis of Chlamydia trachomatis FLAG®-tagged IncA (blue) in HeLa cells. Cells were infected with a GFP-expressing strain of Chlamydia trachomatis containing FLAG-IncA under the control of the Tetracycline (Tet) promoter, and either left untreated (0 ng/mL Tet) or treated with anhydrotetracycline (10 ng/mL Tet). Cells were fixed with 2% paraformaldehyde in PBS for 30 minutes at room tempe... View More
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.jpg?time=20240507?time=20240507 "DYKDDDDK Tag Antibody (MA1-91878) in WB")
Product Details
MA1-91878
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
FG4R
Immunogen
Synthetic peptide (DYKDDDDK) coupled to KLH.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects Flag tag from various samples.
similar epitope as the FLAG tag from Sigma
MA1-91878 has been successfully used in immunoprecipitation, Western blot and immunofluorescence applications.
The MA1-91878 immunogen is synthetic peptide (DYKDDDDK) coupled to KLH.
MA1-91878 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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