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Invitrogen
CDK7 Monoclonal Antibody (31TF2-1F8)
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FIGURE: 1 / 9
CDK7 Antibody (MA3-001) in WB
Western blot was performed using Anti-CDK7 Monoclonal Antibody (31TF2-1F8) (Product # MA3-001) and a 39 kDa band corresponding to CDK7 was observed across cell lines and tissue extracts tested along with a 25kDa band which corresponds to free IgG. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), Hep G2 (Lane 2), MCF-7 (Lane 3), HeLa (Lane 4), Jurkat (Lane 5), HCT116 (Lane 6), K-562 (Lane 7), tissue extracts of Mouse Heart (Lane 8), Mouse Testis (Lane 9) and Mouse Ovary (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protei... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA3-001
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Fish,
Human,
Non-human primate,
Pig,
Yeast
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
31TF2-1F8
Immunogen
Synthetic peptide MALDVKSRAKRYEKLDFLGEGQ corresponding to aa 1-22 of human CDK7
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-001 has been used successfully in Western blotting to detect Cyclin dependent kinase 7 in human lysates.
Target Information
The trimeric complex of CDK7, cyclin H and MNAT1, functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This complex is thought to serve as a direct link between the regulation of transcription and the cell cycle. The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). CDK7 may be a target of germline cancer-inducing mutations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 39 KDa protein kinase; CDK; CDK-activating kinase; CDK-activating kinase 1; cell division protein kinase 7; CR4 protein kinase; CRK4 PK (CDC2-related-kinase-4 protein kinase); cyclin-dependent kinase 7 (homolog of Xenopus MO15 cdk-activating kinase); cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase); EC 2.7.11.22; EC 2.7.11.23; homolog of Xenopus MO15 Cdk-activating kinase; kinase subunit of CAK; OTTHUMP00000222032; OTTHUMP00000222033; protein-tyrosine kinase MPK-7; serine/threonine kinase stk1; serine/threonine protein kinase 1; serine/threonine protein kinase MO15; TFIIH basal transcription factor complex kinase subunit; unnamed protein product
Gene Aliases: C230069N13; CAK; CAK1; CDKN7; Crk4; HCAK; MO15; P39 Mo15; p39MO15; STK1
UniProt ID: (Human) P50613, (Mouse) Q03147
Entrez Gene ID: (Human) 1022, (Mouse) 12572
protein kinase activity
protein serine/threonine kinase activity
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
DNA-dependent ATPase activity
RNA polymerase II carboxy-terminal domain kinase activity
kinase activity
protein serine kinase activity
RNA polymerase II CTD heptapeptide repeat S5 kinase activity
macromolecular complex binding
nucleotide-excision repair
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
snRNA transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
protein stabilization
regulation of cell cycle
regulation of G1/S transition of mitotic cell cycle
promoter clearance from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
protein phosphorylation
positive regulation of transcription elongation from RNA polymerase II promoter
proteasome-mediated ubiquitin-dependent protein catabolic process
transcription pausing by RNA polymerase II
RNA polymerase II transcription initiation surveillance
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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