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Invitrogen
HSP27 Monoclonal Antibody (G3.1)
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FIGURE: 1 / 15
HSP27 Antibody (MA3-015) in ICC/IF
Immunofluorescent analysis of Heat Shock Protein 27 (Hsp27, green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with (left panel) or without (right panel) a Hsp27 monoclonal antibody (Product # MA3-015), at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody (Product # ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA3-015
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Non-human primate,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
G3.1
Immunogen
Partially purified human HSP27.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-015 detects heat shock protein 27 kDa (HSP27) from human, rat, mouse, monkey, and canine samples.
MA3-015 has been successfully used in Western blot, immunohistochemistry (paraffin), immunoprecipitation, immunofluorescence, immunocytochemistry and ELISA procedures. By Western blot, this antibody detects an ~24 kDa protein representing HSP27 in MCF-7 cell extract. Immunohistochemical staining of human cervix with MA3-015 results in intense cytoplasmic staining of the epithelium within the parabasal cell layer. Western blot detection of HSP27 in canine heart samples is weak and may require optimization.
The MA3-015 antigen is partially purified HSP27 derived from MCF-7 cytosol. The mouse HSP27 sequence is strongly conserved within the human sequence, so cross-reactivity with mouse HSP27 is expected.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
Target Information
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 27-kda heat shock protein; 28 kDa heat shock protein; DKFZp586P1322; epididymis secretory protein Li 102; estrogen-regulated 24 kDa protein; heat shock 27 kDa protein; heat shock 27 kDa protein 1; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; heat shock protein 27 kDa beta-1; heat shock protein B1; heat shock protein family B member 1; HS; HSP; HSP 27; HSP27; HSPB1; Phospho-HSP 27; stress-responsive protein 27; unnamed protein product
Gene Aliases: CMT2F; HEL-S-102; HMN2B; HMND3; HS.76067; Hsp25; HSP27; HSP28; SRP27
UniProt ID: (Human) P04792
Entrez Gene ID: (Human) 3315, (Dog) 403979, (Rat) 24471
RNA binding
protein kinase C binding
protein binding
protein kinase C inhibitor activity
protein kinase binding
identical protein binding
protein homodimerization activity
ubiquitin binding
protein binding involved in protein folding
RNA polymerase II sequence-specific DNA binding transcription factor binding
regulation of protein phosphorylation
response to ischemia
protein folding
female pregnancy
response to heat
response to virus
positive regulation of neuron projection development
positive regulation of interleukin-1 beta production
positive regulation of tumor necrosis factor production
intracellular signal transduction
cellular response to vascular endothelial growth factor stimulus
response to muscle stretch
protein refolding
negative regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of blood vessel endothelial cell migration
negative regulation of regulatory T cell differentiation
positive regulation of angiogenesis
vascular endothelial growth factor receptor signaling pathway
protein stabilization
intestinal epithelial structure maintenance
cellular response to hydrogen peroxide
cellular response to interleukin-11
negative regulation of protein kinase C signaling
anterograde axonal protein transport
negative regulation of cellular response to oxidative stress
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
cellular response to butyrate
response to angiotensin
positive regulation of endothelial cell chemotaxis
negative regulation of apoptotic signaling pathway
regulation of translational initiation
response to unfolded protein
regulation of autophagy
platelet aggregation
chaperone-mediated protein folding
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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