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Invitrogen
Vinculin Monoclonal Antibody (VLN01)
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FIGURE: 1 / 27
Vinculin Antibody (MA5-11690) in ICC/IF
Immunofluorescence analysis of Vinculin was performed using 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Vinculin (VLN01) Mouse Monoclonal Antibody (Product # MA5-11690) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conj µgate (Pro... View More
Product Details
MA5-11690
Applications
Tested Dilution
Publications
Product Specifications
Published species
Cat,
Human,
Mouse,
Zebrafish
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
VLN01
Immunogen
Semi-purified vinculin
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-11690 targets Vinculin in IF, IHC (P), IP, and WB applications and shows reactivity with Human samples.
The MA5-11690 immunogen is semi-purified vinculin.
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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