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Invitrogen
PIK3CA Monoclonal Antibody (H.843.0)
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FIGURE: 1 / 6
PIK3CA Antibody (MA5-14870) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of Jurkat (Lane 1), HeLa (Lane 2), U-87 MG (Lane 3), SH-SY5Y (Lane 4) and A-431 (Lane 5). The blot was probed with Anti-PIK3CA Monoclonal Antibody (Product # MA5-14870, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 110 kDa band corresponding to PIK3CA was observed across the cell lines tested.
Product Details
MA5-14870
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
H.843.0
Immunogen
Synthetic peptide surrounding Asp520 of the sequence of human PI3K p110a
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
121 µg/mL
Amount
12.1 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 2.7.1.153; EC:2.7.11.1; GRB1; MGC142161; MGC142163; mutant phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; p110alpha; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; Phosphoinositide 3-kinase alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; phosphoinositide-3-kinase, catalytic, alpha polypeptide; PI3-kinase p110 subunit alpha; PI3-kinase subunit alpha; PI3K; PI3K alpha; PI3K-alpha; PI3Kalpha; pi3kca; PIK3R1; pk3ca; ptdIns-3-kinase p110; ptdIns-3-kinase subunit alpha; PtdIns-3-kinase subunit p110-alpha; Serine/threonine protein kinase PIK3CA; unnamed protein product
Gene Aliases: 6330412C24Rik; caPI3K; CCM4; CLAPO; CLOVE; CWS5; HMH; MCAP; MCM; MCMTC; p110; p110-alpha; p110alpha; PI3K; PI3K-alpha; PIK3CA
UniProt ID: (Bovine) P32871, (Human) P42336, (Mouse) P42337, (Rat) A0A0G2K344
Entrez Gene ID: (Bovine) 282306, (Human) 5290, (Mouse) 18706, (Rat) 170911
protein serine/threonine kinase activity
protein binding
ATP binding
protein kinase activator activity
phosphatidylinositol 3-kinase activity
insulin receptor substrate binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
phosphatidylinositol-3,4-bisphosphate 5-kinase activity
nucleotide binding
kinase activity
1-phosphatidylinositol-3-kinase activity
transferase activity
1-phosphatidylinositol-4-phosphate 3-kinase activity
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
phosphotransferase activity, alcohol group as acceptor
kinase
angiogenesis
liver development
glucose metabolic process
protein phosphorylation
phagocytosis
regulation of gene expression
activation of protein kinase activity
positive regulation of peptidyl-serine phosphorylation
regulation of multicellular organism growth
regulation of cellular respiration
protein kinase B signaling
negative regulation of neuron apoptotic process
hypomethylation of CpG island
phosphatidylinositol phosphorylation
adipose tissue development
cellular response to glucose stimulus
energy homeostasis
negative regulation of fibroblast apoptotic process
regulation of genetic imprinting
negative regulation of anoikis
vasculature development
lipid metabolic process
epidermal growth factor receptor signaling pathway
insulin receptor signaling pathway
positive regulation of lamellipodium assembly
negative regulation of gene expression
response to activity
response to muscle inactivity
negative regulation of macroautophagy
cell migration
actin cytoskeleton organization
platelet activation
negative regulation of actin filament depolymerization
positive regulation of TOR signaling
cellular response to insulin stimulus
response to muscle stretch
phosphatidylinositol-3-phosphate biosynthetic process
vascular endothelial growth factor signaling pathway
response to leucine
anoikis
endothelial cell migration
insulin-like growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
positive regulation of smooth muscle cell proliferation
T cell receptor signaling pathway
positive regulation of protein kinase B signaling
relaxation of cardiac muscle
cardiac muscle contraction
cellular response to hydrostatic pressure
response to dexamethasone
cardiac muscle cell contraction
regulation of actin filament organization
autosome genomic imprinting
response to butyrate
positive regulation of protein localization to membrane
adaptive immune response
inflammatory response
phosphatidylinositol 3-kinase signaling
phosphorylation
innate immune response
positive regulation of protein kinase activity
cell chemotaxis
mitophagy in response to mitochondrial depolarization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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