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Invitrogen
Caspase 8 Monoclonal Antibody (B.925.8)
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FIGURE: 1 / 7
Caspase 8 Antibody (MA5-15226) in WB
Western blot analysis of Caspase 8 was performed by loading 30 ug of HeLa WT untreated (Lane 1), HeLa WT Staurosporine treated (Lane 2), HeLa Cas9 untreated (Lane 3), HeLa Cas9 Staurosporine treated (Lane 4), HeLa Caspase 8 knockout untreated (Lane 5) and HeLa Caspase 8 knockout Staurosporine treated (Lane 6) whole cell lysate. The blot was probed with Caspase 8 Monoclonal Antibody (B.925.8) (Product # MA5-15226, 1:500 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml 1:400... View More
Product Details
MA5-15226
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
B.925.8
Immunogen
Synthetic peptide corresponding to the carboxy-terminal sequence of the p18 fragment of human caspase-8
Conjugate
Form
Liquid
Concentration
619 µg/mL
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other caspases.
Target Information
Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases play a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a pro-domain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. Caspase 8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of Caspase 8 suggests that it may interact with Fas-interacting protein FADD. Caspase 8 was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the pro-form of the Caspase 8 protein. Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby committing the cell to undergo apoptosis. In addition, Caspase 8 also reacts with Jurkat cells and Tonsil. Overexpression of Caspase 8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. Many alternatively spliced transcript variants encoding different isoforms have been described for Caspase 8, however, not all variants have had their full-length sequences determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; CASP-8; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; caspase precursor; Caspase-8; Caspase-8 precursor; Caspase8; EC 3.4.22.61; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE-like apoptotic protease 5; ICE8; MACH; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MGC78473; MORT1-associated ced-3 homolog; OTTHUMP00000163720; OTTHUMP00000165063; OTTHUMP00000206557; OTTHUMP00000206581
Gene Aliases: ALPS2B; CAP4; Casp-8; CASP8; FLICE; MACH; MCH5
UniProt ID: (Human) Q14790
Entrez Gene ID: (Human) 841
cysteine-type endopeptidase activity
death receptor binding
tumor necrosis factor receptor binding
protein binding
peptidase activity
cysteine-type peptidase activity
hydrolase activity
ubiquitin protein ligase binding
death effector domain binding
identical protein binding
macromolecular complex binding
scaffold protein binding
autophagosome assembly
angiogenesis
regulation of cytokine production
regulation of immune system process
proteolysis
apoptotic process
heart development
extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of signal transduction
regulation of tumor necrosis factor-mediated signaling pathway
protein processing
natural killer cell activation
cell differentiation
macrophage differentiation
positive regulation of cell migration
response to cobalt ion
response to estradiol
response to lipopolysaccharide
positive regulation of interleukin-1 beta production
response to tumor necrosis factor
TRAIL-activated apoptotic signaling pathway
T cell activation
B cell activation
regulation of apoptotic process
chordate embryonic development
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of neuron apoptotic process
regulation of innate immune response
response to ethanol
positive regulation of macrophage differentiation
positive regulation of proteolysis
animal organ development
proteolysis involved in cellular protein catabolic process
protein maturation
negative regulation of necroptotic process
syncytiotrophoblast cell differentiation involved in labyrinthine layer development
pyroptosis
cellular response to mechanical stimulus
response to anesthetic
apoptotic signaling pathway
extrinsic apoptotic signaling pathway
execution phase of apoptosis
self proteolysis
positive regulation of pyroptotic inflammatory response
positive regulation of execution phase of apoptosis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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