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Invitrogen
ASK1 Monoclonal Antibody (2E4)
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Product Details
MA5-15861
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2E4
Immunogen
Purified recombinant fragment of human MAP3K5 expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-15861 targets MAP3K5 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15861 immunogen is purified recombinant fragment of human MAP3K5 expressed in E. Coli. .
MA5-15861 detects MAP3K5 which has a predicted molecular weight of approximately 55kDa.
Target Information
MAP3K5 (ASK1) is a serine/threonine kinase that is abundantly expressed in human heart and pancreas and are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activates c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death and a catalytically inactive form of ASK1 inhibited TNF-a-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apoptosis signal-regulating kinase 1; ASK 1; ASK-1; EC 2.7.11.25; kinase ASK1; M3K5; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; Mitogen-activated protein kinase kinase kinase 5; RP3-325F22.4; unnamed protein product
Gene Aliases: ASK1; MAP3K5; MAPKKK5; MEKK5
UniProt ID: (Human) Q99683
Entrez Gene ID: (Human) 4217
nucleotide binding
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
JUN kinase kinase kinase activity
MAP kinase kinase kinase activity
protein binding
ATP binding
kinase activity
transferase activity
protein kinase binding
protein phosphatase binding
protein domain specific binding
identical protein binding
protein homodimerization activity
metal ion binding
protein serine kinase activity
MAPK cascade
immune system process
response to ischemia
apoptotic process
JNK cascade
intrinsic apoptotic signaling pathway in response to oxidative stress
positive regulation of cardiac muscle cell apoptotic process
cellular response to amino acid starvation
response to endoplasmic reticulum stress
neuron intrinsic apoptotic signaling pathway in response to oxidative stress
p38MAPK cascade
positive regulation of apoptotic process
regulation of programmed cell death
positive regulation of MAPK cascade
innate immune response
positive regulation of myoblast differentiation
positive regulation of transcription, DNA-templated
positive regulation of JNK cascade
neuron apoptotic process
stress-activated MAPK cascade
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to hydrogen peroxide
cellular response to tumor necrosis factor
endothelial cell apoptotic process
cellular senescence
apoptotic signaling pathway
programmed necrotic cell death
positive regulation of p38MAPK cascade
cellular response to reactive nitrogen species
positive regulation of vascular smooth muscle cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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