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Invitrogen
HSP27 Monoclonal Antibody (G3.1), Biotin
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FIGURE: 1 / 4
HSP27 Antibody (MA5-11213) in WB
Knockdown of HSP27 / Heat Shock Protein 27 was achieved by transfecting HeLa cells with HSP27 / Heat Shock Protein 27 specific siRNAs (Silencer® select Product # s194537 and s6991). Western blot analysis (Fig a) was performed using whole cell extract from the HSP27 / Heat Shock Protein 27 knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-HSP27 / Heat Shock Protein 27 Biotin Mouse Monoclonal Antibody (Product # MA5-11213, 1ug/mL) and Poly-HRP ... View More
Product Details
MA5-11213
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
G3.1
Immunogen
Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
MA5-11213 targets Heat Shock Protein 27 in IHC (P) and WB applications and shows reactivity with Human and Non-human primate samples.
The MA5-11213 immunogen is partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
Target Information
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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