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Invitrogen
Cytokeratin 14 Monoclonal Antibody (LL002), Biotin
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FIGURE: 1 / 3
Cytokeratin 14 Antibody (MA5-11596) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), Hep G2 (Lane 2), tissue extracts (30 µg lysate) of Mouse Skin (Lane 3) and Mouse Liver (Lane 4). The blot was probed with Mouse Anti-Cytokeratin 14 Monoclonal Antibody (Product # MA5-11596, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 52 kDa band corresponding to Cytokeratin 14 was observed in A-431 and Mouse Skin, but not... View More
Product Details
MA5-11596
Applications
Tested Dilution
Publications
Product Specifications
Published species
Cat,
Dog,
Human,
Mouse,
Rat,
Rhesus monkey
Host/Isotype
Mouse
/ IgG3
Class
Monoclonal
Type
Antibody
Clone
LL002
Immunogen
A synthetic peptide of 15 amino acid residues from the C-terminus of human keratin 14
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
MA5-11596 targets Cytokeratin 14 in IHC (P) and WB applications and shows reactivity with Human and Rat samples.
The MA5-11596 immunogen is a synthetic peptide of 15 amino acid residues from the C-terminus of human keratin 14.
Target Information
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie. skin) and non-keratinizing cells (ie. mesothelial cells). At least 20 different CKs can be identified. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). Belonging to the type I subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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