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Invitrogen
Cytochrome C Monoclonal Antibody (7H8.2C12)
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FIGURE: 1 / 18
Cytochrome C Antibody (MA5-11674) in ICC/IF
Immunofluorescence analysis of Cytochrome was performed using 70 % confluent log phase HeLa cells. The cells were fixed with 4 % paraformaldehyde for 10 minutes, permeabilized with 0.1 % Triton™ X-100 for 15 minutes, and blocked with 2 % BSA for 45 minutes at room temperature. The cells were labeled with Cytochrome C Monoclonal Antibody (7H8.2C12) (Product # MA5-11674) at 0.66 µg/mL in 0.1 % BSA, incubated ... View More
Product Details
MA5-11674
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Fruit fly,
Human,
Mouse,
Rat,
Xenopus
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
7H8.2C12
Immunogen
Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon cytochrome c
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-11674 targets Cytochrome c in IHC (P) and WB applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-11674 immunogen is synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon cytochrome c.
Target Information
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CRF; CYCS; Cytochrome c; Cytochrome c, somatic; hc1; unnamed protein product
Gene Aliases: CYC; CYCS; HCS; THC4
UniProt ID: (Human) P99999, (Mouse) P62897
Entrez Gene ID: (Human) 54205, (Mouse) 13063
mitochondrial electron transport, ubiquinol to cytochrome c
mitochondrial electron transport, cytochrome c to oxygen
apoptotic process
cellular respiration
apoptotic signaling pathway
intrinsic apoptotic signaling pathway
execution phase of apoptosis
apoptotic DNA fragmentation
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
hydrogen peroxide metabolic process
ATP synthesis coupled electron transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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