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Invitrogen
XPF Monoclonal Antibody (51)
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FIGURE: 1 / 7
XPF Antibody (MA5-12060) in WB
Western blot analysis was performed on nuclear extracts (30 µg lysate) of A-375 (Lane 1), A-431 (Lane 2), SK-OV-3 (Lane 3), Sf9 (Lane 4), Hep G2 (Lane 5), HeLa (Lane 6), A549 (Lane 7) and Jurkat (Lane 8). The blot was probed with Anti-XPF Mouse Monoclonal Antibody (Product # MA5-12060, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 104 kDa band corresponding to XPF was observed across the cell lines tested. Known quan... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-12060
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
51
Immunogen
Recombinant human XPF protein
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-12060 targets XPF in WB applications and shows reactivity with Human samples.
The MA5-12060 immunogen is recombinant human XPF protein.
Target Information
The structure specific ERCC 1 / XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells, XPG cleaves 3' of the DNA lesion while the ERCC1 / XPF complex makes the 5' incision. ERCC1 / XPF probably plays a role in processing partially processed recombination intermediates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DNA excision repair protein ERCC-4; DNA repair protein complementing XP-F cells; excision repair cross-complementation group 4; excision repair cross-complementing rodent repair deficiency, complementation group 4; excision-repair, complementing defective, in Chinese hamster; unnamed protein product; xeroderma pigmentosum group F-complementing protein; xeroderma pigmentosum, complementation group F
Gene Aliases: ERCC11; FANCQ; RAD1; XFEPS; XPF
UniProt ID: (Human) Q92889
Entrez Gene ID: (Human) 2072
single-stranded DNA endodeoxyribonuclease activity
TFIID-class transcription factor binding
DNA binding
damaged DNA binding
single-stranded DNA binding
endodeoxyribonuclease activity
protein binding
telomerase inhibitor activity
protein binding, bridging
identical protein binding
3' overhang single-stranded DNA endodeoxyribonuclease activity
promoter-specific chromatin binding
resolution of meiotic recombination intermediates
telomere maintenance
double-strand break repair via homologous recombination
DNA repair
nucleotide-excision repair
double-strand break repair via nonhomologous end joining
mitotic recombination
response to UV
negative regulation of telomere maintenance
cellular response to UV
telomeric DNA-containing double minutes formation
nucleotide-excision repair involved in interstrand cross-link repair
negative regulation of telomere maintenance via telomere lengthening
negative regulation of protection from non-homologous end joining at telomere
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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