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          • Primary Antibodies ›
          • Her-2 (ErbB2) Antibodies
          The product (cat # {{productObj.productId}}) that you're looking for has been discontinued and replaced with {{productObj.replacementProductId}}. The product (cat # {{productObj.productId}}) that you're looking for has been discontinued. Please contact Technical Support with any questions.

          Invitrogen

          ErbB2 (HER-2) Monoclonal Antibody (e2-4001), Biotin

          Advanced Verification
          3 Published Figures
          89 References
          View all (230) Her-2 (ErbB2) antibodies

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          Cite ErbB2 (HER-2) Monoclonal Antibody (e2-4001), Biotin

          Additional Information:
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          • Antibody Testing Data (4)
          • Published Figures (3)
          • Advanced Verification (1)
          ErbB2 (HER-2) Antibody in Western Blot (WB)
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          ErbB2 (HER-2) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          ErbB2 (HER-2) Antibody (MA5-13102) in WB

          Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of SK-BR-3 (1) and MDA-MB-231 (2). The blots were probed with Anti-ErbB2 Mouse Monoclonal Antibody (Product # MA5-13102, 1 µg/mL dilution) and detected by chemiluminescence using Streptavidin-HRP (Product # N200, 1:10,000 dilution). A 185 kDa band corresponding to ErbB2 was observed in SK-BR-3 but not in MDA-MB-231, which is a negative control for ErbB2. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          ErbB2 (HER-2) Antibody in Western Blot (WB)
          ErbB2 (HER-2) Antibody in Western Blot (WB)
          ErbB2 (HER-2) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ErbB2 (HER-2) Antibody in Immunoprecipitation (IP)
          ErbB2 (HER-2) Antibody in Immunocytochemistry (ICC/IF)
          ErbB2 (HER-2) Antibody in Immunocytochemistry (ICC/IF)
          ErbB2 (HER-2) Antibody in Immunocytochemistry (ICC/IF)
          ErbB2 (HER-2) Antibody

          Product Details

          MA5-13102

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          View 24 publications 24 publications

          Immunohistochemistry (IHC)

          -
          View 51 publications 51 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200-1:400
          -

          Immunocytochemistry (ICC/IF)

          -
          View 6 publications 6 publications

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          2 µg/mL
          View 6 publications 6 publications

          Gel Shift (EMSA)

          -
          View 1 publication 1 publication
          Product Specifications

          Published species

          Cat, Hamster, Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          e2-4001

          Immunogen

          Cytoplasmic domain of recombinant human c-erbB-2/HER-2 oncoprotein

          Conjugate

          Biotin Biotin Biotin

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Amount

          100 µg

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10984470

          Product Specific Information

          MA5-13102 targets HER-2 in IHC, IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.

          The MA5-13102 immunogen is cytoplasmic domain of recombinant human c-erbB-2/HER-2 oncoprotein.

          Target Information

          ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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