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Invitrogen
Cyclin B1 Monoclonal Antibody (V152)
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FIGURE: 1 / 15
Cyclin B1 Antibody (MA5-13128) in ICC/IF
Immunofluorescence analysis of Cyclin B1 was performed using 70% confluent log phase HT-29 cells treated 100 ng of Nocodazole for 16 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cyclin B1 Mouse (V152) Monoclonal Antibody (Product # MA5-13128) at 2µ... View More
Product Details
MA5-13128
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
V152
Immunogen
Hamster cyclin B1 protein
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Amount
50 µg
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-13128 targets Cyclin B1 in FACS, IHC (P), and WB applications and shows reactivity with Hamster and Human samples.
The MA5-13128 immunogen is hamster cyclin B1 protein.
Target Information
Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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