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Invitrogen
Actin Muscle Monoclonal Antibody (HHF35)
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FIGURE: 1 / 5
Actin Muscle Antibody (MA5-14084) in ICC/IF
Immunofluorescent analysis of Actin Muscle Specific (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Actin Muscle Specific monoclonal antibody (Product # ... View More
Product Details
MA5-14084
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
HHF35
Immunogen
SDS extract of human myocardium
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
tissue culture supernatant
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with actin from tissue extracts of uterus, ileum, aorta, diaphragm, heart and smooth muscle cells. It recognizes alpha-actin from skeletal, cardiac, and smooth muscle, and reacts with gamma actin from smooth muscle. This antibody will react with tumors from smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle tumors (rhabdomyomas and rhabdomyosarcomas).
Staining of formalin-fixed tissues requires boiling in 1mM EDTA, pH 8.0, followed by cooling at room temperature for 20 min.
Target Information
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ACTA1; ACTA2; ACTC; ACTG2; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, cytoplasmic 2; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; actin-like protein; alpha-actin; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; beta-actin FE-3; Cell growth-inhibiting gene 46 protein; cytoskeletal gamma-actin; epididymis luminal protein 176; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; GIG46; nemaline myopathy type 3; RP5-1068B5.2; smooth muscle alpha-actin; Smooth muscle gamma-actin; unnamed protein product
Gene Aliases: 0610041G09Rik; a-SMA; AA959943; ACT; Act-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; AL023024; alphaSMA; ASD5; ASMA; CFTD; CFTD1; CFTDM; CMD1R; CMH11; CMYO2A; CMYO2B; CMYO2C; CMYP2A; CMYP2B; CMYP2C; DFNA20; DFNA26; E51; GIG46; HEL-176; LVNC4; MMIHS5; MPFD; NEM1; NEM2; NEM3; SHPM; SMalphaA; SMDYS; SMGA; VSCM; VSCM1
UniProt ID: (Human) P68133, (Human) P62736, (Human) P68032, (Human) P63261, (Human) P63267, (Mouse) P68134, (Rat) P68136, (Rat) P62738, (Mouse) P62737, (Mouse) P68033, (Rat) P68035, (Mouse) P63260, (Rat) P63259, (Mouse) P63268, (Rat) P63269
Entrez Gene ID: (Human) 58, (Human) 59, (Human) 70, (Human) 71, (Human) 72, (Mouse) 11459, (Rat) 29437, (Rat) 81633, (Mouse) 11475, (Mouse) 11464, (Rat) 29275, (Mouse) 11465, (Rat) 287876, (Mouse) 11468, (Rat) 25365
nucleotide binding
structural constituent of cytoskeleton
protein binding
ATP binding
hydrolase activity
myosin binding
ADP binding
protein kinase binding
microfilament motor activity
profilin binding
ubiquitin protein ligase binding
identical protein binding
structural constituent of postsynaptic actin cytoskeleton
ATPase activity
molecular_function
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
muscle contraction
positive regulation of gene expression
skeletal muscle thin filament assembly
skeletal muscle fiber development
mesenchyme migration
regulation of blood pressure
response to virus
vascular smooth muscle contraction
positive regulation of hepatic stellate cell migration
positive regulation of hepatic stellate cell contraction
positive regulation of ERK1 and ERK2 cascade
cellular response to transforming growth factor beta stimulus
juxtaglomerular apparatus development
glomerular mesangial cell development
positive regulation of hepatic stellate cell activation
actin filament organization
response to xenobiotic stimulus
actin filament-based movement
actomyosin structure organization
actin-myosin filament sliding
negative regulation of apoptotic process
response to ethanol
cardiac myofibril assembly
cardiac muscle tissue morphogenesis
heart contraction
cardiac muscle contraction
cytoplasmic actin-based contraction involved in cell motility
angiogenesis
morphogenesis of a polarized epithelium
axonogenesis
positive regulation of cell migration
maintenance of blood-brain barrier
sarcomere organization
cell motility
regulation of stress fiber assembly
regulation of focal adhesion assembly
platelet aggregation
cellular response to interferon-gamma
positive regulation of wound healing
postsynaptic actin cytoskeleton organization
tight junction assembly
regulation of transepithelial transport
regulation of synaptic vesicle endocytosis
protein localization to bicellular tight junction
response to mechanical stimulus
response to extracellular stimulus
response to lithium ion
cell growth
skeletal muscle fiber adaptation
response to steroid hormone
metabolic process
actin-mediated cell contraction
response to drug
retina homeostasis
response to calcium ion
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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