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Invitrogen
Estrogen Receptor alpha Antibody Cocktail
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FIGURE: 1 / 6
Estrogen Receptor alpha Antibody (MA5-14104) in ICC/IF
Immunofluorescent analysis of Estrogen Receptor alpha (ER alpha, green) in T47D and Hs 578T breast cancer cells. The cells were fixed and permeabilized with ice-cold methanol for 10 minutes at room temperature, and blocked with 0.3% BSA in PBS for at least 30 minutes at room temperature. Cells were stained with an ER alpha monoclonal antibody (Product # MA5-14104) at a dilution of 1:100 in blocking buffer o... View More
Product Details
MA5-14104
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Cat,
Chicken,
Human,
Mouse,
Rabbit,
Rat,
Rhesus monkey
Host/Isotype
Mouse
/ IgG1
Class
Cocktail
Type
Antibody
Clone
1D5, 6F11
Immunogen
Recombinant human estrogen receptor protein
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
tissue culture supernatant
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-14104 is a mixture of two monoclonal antibodies that target Estrogen Receptor alpha.
Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissues in 10mM citrate buffer (pH 6.0) for 10-20 min, followed by cooling at room temperature for 20 min. A recommended positive control is MCF-7 cells.
Target Information
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp686N23123; E2 receptor alpha; ER; ER delta E7 isoform; ER-alpha; ER-alpha; del.3*,+1*,4,5,6,7* variant; ER-alpha; del.3*,4,5,6,7*/770 variant; ER-alpha; del.3*,4,5,6,7,8*/862 variant; ER-alpha; del.4*,5*,7 variant; ER-alpha; del.4*,5,6*/548 variant; ER-alpha; del.4*,5,6,7*/714 variant; ER-alpha; del.4*,5,6,7,8*/754 variant; ER-alpha; estrogen receptor alpha delta 3*,4,5,6,7*/819 cingulate gyrus isoform; ER-alpha; estrogen receptor alpha delta 3*,4,5,6,7*/833 superior frontal gyrus isoform; ER-alpha; estrogen receptor alpha delta 4*,5,6,7*/747 substantia nigra isoform; ER36; esr 1; esr-1; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor-related protein; hER-alpha36; lacks a part of the exon 3, exons 4-6, and part of exon 7; contains an 822 base in frame deletion; lacks most of the exon 3, exons 4-7, and part of exon 8; lacks most of the exon 4, exons 5-6, and part of exon 7; lacks the end of the exon 3, exons 4-7, and part of exon 8; MB1; Nuclear receptor subfamily 3 group A member 1; oestrogen receptor alpha; receptor; RP1-130E4.1; steroid hormone receptor; unnamed protein product
Gene Aliases: ER; Era; ESR; ESR1; ESRA; ESTRR; NR3A1
UniProt ID: (Human) P03372
Entrez Gene ID: (Human) 2099
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
TFIIB-class transcription factor binding
transcription cofactor binding
transcription corepressor binding
transcription coactivator binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
steroid binding
protein binding
calmodulin binding
beta-catenin binding
zinc ion binding
lipid binding
TBP-class protein binding
enzyme binding
protein kinase binding
nitric-oxide synthase regulator activity
estrogen receptor activity
estrogen receptor binding
estrogen response element binding
identical protein binding
sequence-specific DNA binding
metal ion binding
ATPase binding
14-3-3 protein binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
antral ovarian follicle growth
epithelial cell development
chromatin remodeling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
signal transduction
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
androgen metabolic process
male gonad development
negative regulation of gene expression
intracellular steroid hormone receptor signaling pathway
intracellular estrogen receptor signaling pathway
response to estradiol
regulation of toll-like receptor signaling pathway
negative regulation of smooth muscle cell apoptotic process
negative regulation of I-kappaB kinase/NF-kappaB signaling
steroid hormone mediated signaling pathway
negative regulation of sequence-specific DNA binding transcription factor activity
response to estrogen
positive regulation of nitric oxide biosynthetic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
fibroblast proliferation
positive regulation of fibroblast proliferation
stem cell differentiation
regulation of inflammatory response
positive regulation of nitric-oxide synthase activity
positive regulation of sequence-specific DNA binding transcription factor activity
RNA polymerase II transcriptional preinitiation complex assembly
uterus development
vagina development
prostate epithelial cord elongation
prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis
regulation of branching involved in prostate gland morphogenesis
mammary gland branching involved in pregnancy
mammary gland alveolus development
epithelial cell proliferation involved in mammary gland duct elongation
protein localization to chromatin
cellular response to estrogen stimulus
cellular response to estradiol stimulus
cellular response to oxygen-containing compound
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
regulation of epithelial cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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