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Invitrogen
Cytokeratin 5 Monoclonal Antibody (EP1601Y)
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FIGURE: 1 / 15
Cytokeratin 5 Antibody (MA5-14473) in ICC/IF
Immunofluorescence analysis of Cytokeratin 5 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 5 Monoclonal Antibody (Product # MA5-14473) at 5µg/mL in 0.1% BSA and incubated for 3 hours at r... View More
Product Details
MA5-14473
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
EP1601Y
Immunogen
A synthetic peptide corresponding to residues on the C-term of human CK5
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
MA5-14473 targets Cytokeratin 5 in IHC (P) applications and shows reactivity with Human samples.
The MA5-14473 immunogen is a synthetic peptide corresponding to residues on the C-term of human CK5.
Target Information
Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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