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Invitrogen
ApoA1 Monoclonal Antibody (6001)
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FIGURE: 1 / 5
ApoA1 Antibody (MA5-14667) in IHC (P)
Immunohistochemistry was performed on human liver tissue sections. To expose Apo A-1, tissues were microwaved for 10 minutes in 10mM sodium citrate, pH 6.0. Following antigen retrieval, endogenous peroxidases were blocked in a 3% hydrogen peroxide-methanol solution for 15 minutes, and then tissues were blocked in 3% BSA in PBS for 30 minutes at room temperature. Tissues were probed with an Apo A-1 monoclona... View More
Product Details
MA5-14667
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
6001
Immunogen
Purified human plasma Apo A-1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Ion-exchange chromatography
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
By sandwich ELISA, MA5-14667 can be used as a detection antibody with Product # 710263 as the coating antibody, to generate a matched pair. Using this matched pair, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. It is not recommended to use Product # 701239 for coating when MA5-14667 is used for detection. By sandwich ELISA, MA5-14667 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MA5-14667, a biotinylated detection antibody followed by Streptavidin-HRP is recommended.
By Western blot, MA5-14667 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MA5-14667 may not be as successful for detecting endogenous Apo A-1 by Western blot.
Product MA514667 is a smaller package size of MIA1401 (formerly sold as a Seradyn product).
Target Information
Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 1-Sep; 2-Sep; Apo a; apo A-1; APO A1; APO I; Apo-AI; ApoAI; Apolipoprotein; Apolipoprotein A-I; Apolipoprotein A1; Apolipoprotein AI; epididymis secretory sperm binding protein; MGC102525; MGC117399; MGC124929; proapo-A-I protein; proapolipoprotein; Udpgt; Ugt1; Ugt1a7; unnamed protein product
Gene Aliases: AMYLD3; apo(a); APOA1; HPALP2
UniProt ID: (Human) P02647
Entrez Gene ID: (Human) 335
beta-amyloid binding
receptor binding
lipid transporter activity
protein binding
phospholipid binding
high-density lipoprotein particle binding
lipid binding
cholesterol binding
enzyme binding
heat shock protein binding
apolipoprotein receptor binding
apolipoprotein A-I receptor binding
identical protein binding
protein homodimerization activity
chemorepellent activity
receptor agonist activity
phosphatidylcholine-sterol O-acyltransferase activator activity
high-density lipoprotein particle receptor binding
lipoprotein particle binding
intermembrane cholesterol transfer activity
endothelial cell proliferation
negative regulation of cytokine production involved in immune response
lipid metabolic process
phospholipid metabolic process
phosphatidylcholine biosynthetic process
cholesterol biosynthetic process
lipid transport
transforming growth factor beta receptor signaling pathway
G-protein coupled receptor signaling pathway
integrin-mediated signaling pathway
steroid metabolic process
cholesterol metabolic process
glucocorticoid metabolic process
negative regulation of tumor necrosis factor-mediated signaling pathway
positive regulation of cholesterol efflux
negative regulation of very-low-density lipoprotein particle remodeling
protein oxidation
peptidyl-methionine modification
lipid storage
regulation of intestinal cholesterol absorption
cholesterol transport
adrenal gland development
regulation of Cdc42 protein signal transduction
negative regulation of interleukin-1 beta production
cholesterol efflux
phospholipid efflux
negative regulation of heterotypic cell-cell adhesion
beta-amyloid formation
high-density lipoprotein particle remodeling
high-density lipoprotein particle assembly
high-density lipoprotein particle clearance
positive regulation of Rho protein signal transduction
lipoprotein metabolic process
lipoprotein biosynthetic process
cholesterol homeostasis
blood vessel endothelial cell migration
reverse cholesterol transport
negative regulation of inflammatory response
positive regulation of phagocytosis
protein stabilization
negative chemotaxis
vitamin transport
positive regulation of stress fiber assembly
acylglycerol homeostasis
phospholipid homeostasis
negative regulation of cell adhesion molecule production
negative regulation of response to cytokine stimulus
triglyceride homeostasis
ERK1 and ERK2 cascade
cholesterol import
cellular response to lipoprotein particle stimulus
positive regulation of cholesterol metabolic process
positive regulation of substrate adhesion-dependent cell spreading
positive regulation of phospholipid efflux
cellular response to beta-amyloid
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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