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Invitrogen

ApoA1 Monoclonal Antibody (6001)

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ApoA1 Monoclonal Antibody (6001)

Product Details

MA5-14667

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

Assay-dependent
-

Radioimmune Assays (RIA)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

6001

Immunogen

Purified human plasma Apo A-1
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Ion-exchange chromatography

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10980499

Product Specific Information

By sandwich ELISA, MA5-14667 can be used as a detection antibody with Product # 710263 as the coating antibody, to generate a matched pair. Using this matched pair, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. It is not recommended to use Product # 701239 for coating when MA5-14667 is used for detection. By sandwich ELISA, MA5-14667 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MA5-14667, a biotinylated detection antibody followed by Streptavidin-HRP is recommended.



By Western blot, MA5-14667 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MA5-14667 may not be as successful for detecting endogenous Apo A-1 by Western blot.



Product MA514667 is a smaller package size of MIA1401 (formerly sold as a Seradyn product).

Target Information

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 1-Sep; 2-Sep; Apo a; apo A-1; APO A1; APO I; Apo-AI; ApoAI; Apolipoprotein; Apolipoprotein A-I; Apolipoprotein A1; Apolipoprotein AI; epididymis secretory sperm binding protein; MGC102525; MGC117399; MGC124929; proapo-A-I protein; proapolipoprotein; Udpgt; Ugt1; Ugt1a7; unnamed protein product

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Gene Aliases: AMYLD3; apo(a); APOA1; HPALP2

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UniProt ID: (Human) P02647

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Entrez Gene ID: (Human) 335

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Function(s)
beta-amyloid binding receptor binding lipid transporter activity protein binding phospholipid binding high-density lipoprotein particle binding lipid binding cholesterol binding enzyme binding heat shock protein binding apolipoprotein receptor binding apolipoprotein A-I receptor binding identical protein binding protein homodimerization activity chemorepellent activity receptor agonist activity phosphatidylcholine-sterol O-acyltransferase activator activity high-density lipoprotein particle receptor binding lipoprotein particle binding intermembrane cholesterol transfer activity
Process(es)
endothelial cell proliferation negative regulation of cytokine production involved in immune response lipid metabolic process phospholipid metabolic process phosphatidylcholine biosynthetic process cholesterol biosynthetic process lipid transport transforming growth factor beta receptor signaling pathway G-protein coupled receptor signaling pathway integrin-mediated signaling pathway steroid metabolic process cholesterol metabolic process glucocorticoid metabolic process negative regulation of tumor necrosis factor-mediated signaling pathway positive regulation of cholesterol efflux negative regulation of very-low-density lipoprotein particle remodeling protein oxidation peptidyl-methionine modification lipid storage regulation of intestinal cholesterol absorption cholesterol transport adrenal gland development regulation of Cdc42 protein signal transduction negative regulation of interleukin-1 beta production cholesterol efflux phospholipid efflux negative regulation of heterotypic cell-cell adhesion beta-amyloid formation high-density lipoprotein particle remodeling high-density lipoprotein particle assembly high-density lipoprotein particle clearance positive regulation of Rho protein signal transduction lipoprotein metabolic process lipoprotein biosynthetic process cholesterol homeostasis blood vessel endothelial cell migration reverse cholesterol transport negative regulation of inflammatory response positive regulation of phagocytosis protein stabilization negative chemotaxis vitamin transport positive regulation of stress fiber assembly acylglycerol homeostasis phospholipid homeostasis negative regulation of cell adhesion molecule production negative regulation of response to cytokine stimulus triglyceride homeostasis ERK1 and ERK2 cascade cholesterol import cellular response to lipoprotein particle stimulus positive regulation of cholesterol metabolic process positive regulation of substrate adhesion-dependent cell spreading positive regulation of phospholipid efflux cellular response to beta-amyloid
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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