Search

  • Contáctenos
  • Orden Rápida

Invitrogen

p21 Monoclonal Antibody (R.229.6)

Advanced Verification
31 Published Figures
40 References
View all (68) p21 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
p21 Monoclonal Antibody (R.229.6)

Product Details

MA5-14949

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 15 publications 15 publications

Immunohistochemistry (IHC)

-
View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:400-1:800
View 15 publications 15 publications

Flow Cytometry (Flow)

1:100-1:400
View 2 publications 2 publications

Immunoprecipitation (IP)

1:50
-
Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

R.229.6

Immunogen

Synthetic peptide corresponding to residues near the carboxy-terminus of human p21
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

244 µg/mL

Amount

24.4 µg

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984461

Product Specific Information

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other CDK inhibitors.

Target Information

Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Activating Fragment 1; alternate gene name=WAF1; CDK-interacting protein 1; CDK-interaction protein 1; CDKN1A; CDNK1A; Cip1); cyclin kinase inhibitor; Cyclin-dependent kinase inhibitor 1; Cyclin-dependent kinase inhibitor 1A (p21; cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein 6; OTTHUMP00000016298; p21; p21Cip1/Waf1; putative DNA synthesis inhibitor; SLC12A9; unnamed protein product; Wild type p53 activated fragment 1 (WAF1); wild type p53 activated fragment-1; wild-type p53-activated fragment 1

View more View less

Gene Aliases: CAP20; CDKN1; CDKN1A; CIP1; MDA-6; MDA6; P21; p21CIP1; PIC1; SDI1; WAF1

View more View less

UniProt ID: (Human) P38936

View more View less

Entrez Gene ID: (Human) 1026

View more View less

Function(s)
protein kinase inhibitor activity cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding zinc ion binding protein kinase binding cyclin binding ubiquitin protein ligase binding macromolecular complex binding metal ion binding protein serine/threonine kinase binding protein sequestering activity molecular function activator activity molecular function inhibitor activity
Process(es)
G1/S transition of mitotic cell cycle in utero embryonic development protein import into nucleus cellular response to DNA damage stimulus mitotic G2 DNA damage checkpoint Ras protein signal transduction regulation of mitotic cell cycle heart development positive regulation of cell proliferation negative regulation of cell proliferation epidermis development response to UV regulation of G2/M transition of mitotic cell cycle negative regulation of macromolecule biosynthetic process negative regulation of gene expression keratinocyte differentiation negative regulation of cell growth DNA damage response, signal transduction by p53 class mediator positive regulation of B cell proliferation mitotic G1 DNA damage checkpoint cellular response to amino acid starvation wound healing tissue regeneration signal transduction in response to DNA damage intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of programmed cell death keratinocyte proliferation positive regulation of DNA replication positive regulation of protein kinase activity negative regulation of growth fibroblast proliferation positive regulation of fibroblast proliferation nuclear import regulation of cell cycle cellular response to cell-matrix adhesion cellular response to ionizing radiation cellular response to gamma radiation cellular response to UV-B signal transduction by p53 class mediator cellular senescence replicative senescence stress-induced premature senescence oncogene-induced cell senescence intrinsic apoptotic signaling pathway regulation of cell cycle G1/S phase transition negative regulation of cyclin-dependent protein kinase activity negative regulation of vascular smooth muscle cell proliferation negative regulation of cardiac muscle tissue regeneration regulation of G1/S transition of mitotic cell cycle negative regulation of G1/S transition of mitotic cell cycle regulation of DNA biosynthetic process negative regulation of DNA biosynthetic process positive regulation of reactive oxygen species metabolic process
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support