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Invitrogen
Phospho-CHK1 (Ser345) Monoclonal Antibody (S.48.4)
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FIGURE: 1 / 8
Phospho-CHK1 (Ser345) Antibody (MA5-15145) in ICC/IF
Immunofluorescence analysis of Phospho-CHK1 (Ser345) was performed using 70% confluent log phase HeLa cells treated with Etoposide (5uM, 16 hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-CHK1 (Ser345) Monoclonal Antibody (S.48.4) (Product # MA... View More
Product Details
MA5-15145
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
C. elegans,
Human
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
S.48.4
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding pSer345 of human Chk1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
37 µg/mL
Amount
3.7 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
CHEK1 (CHK1) is a serine/threonine protein kinase involved in preventing replication when DNA integrity is compromised. CHK1 is a key mediator in the DNA damage-induced checkpoint network. CHK1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. When the G2 or S checkpoint is abrogated by the inhibition of CHK1, p53-deficient cancer cells undergo mitotic catastrophe and eventually apoptosis, whereas normal cells are still arrested in the G1 phase. Thus, CHK1 inhibitors can preferentially potentiate the efficacy of DNA damaging agents in cancer cells, and Chk1 is an attractive therapeutic target for cancer treatment, especially since approximately 50% of all human cancers are p53-deficient.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cell cycle checkpoint kinase; checkpoint kinase 1 homolog; Checkpoint kinase-1; Checkpoint, S. pombe, homolog of, 1; CHK1 checkpoint homolog; Chk1-S; homolog of Chk1-S; HRD1 Protein; rad27 homolog; Serine/threonine-protein kinase Chk1; Synoviolin; unnamed protein product
Gene Aliases: C85740; CHEK1; CHK1; OZEMA21; rad27
UniProt ID: (Human) O14757, (Rat) Q91ZN7, (Mouse) O35280
Entrez Gene ID: (Human) 1111, (Rat) 140583, (Mouse) 12649
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
protein domain specific binding
histone kinase activity (H3-T11 specific)
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
glycosyltransferase
non-receptor serine/threonine protein kinase
DNA damage checkpoint
G2/M transition of mitotic cell cycle
inner cell mass cell proliferation
DNA replication
DNA repair
chromatin remodeling
protein phosphorylation
apoptotic process
cellular response to DNA damage stimulus
nucleus organization
nuclear envelope organization
mitotic nuclear envelope disassembly
mitotic G2 DNA damage checkpoint
regulation of gene expression
regulation of double-strand break repair via homologous recombination
positive regulation of G2/M transition of mitotic cell cycle
peptidyl-threonine phosphorylation
regulation of cell proliferation
signal transduction in response to DNA damage
mitotic G2/M transition checkpoint
positive regulation of cell cycle
negative regulation of gene expression, epigenetic
negative regulation of mitotic nuclear division
regulation of mitotic centrosome separation
negative regulation of G0 to G1 transition
cellular response to mechanical stimulus
replicative senescence
regulation of signal transduction by p53 class mediator
negative regulation of cell cycle phase transition
apoptotic process involved in development
DNA damage induced protein phosphorylation
regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage
negative regulation of G2/M transition of mitotic cell cycle
histone H3-T11 phosphorylation
chromatin-mediated maintenance of transcription
cellular response to organic substance
cellular response to caffeine
negative regulation of DNA biosynthetic process
regulation of histone H3-K9 acetylation
cell cycle
phosphorylation
G2 DNA damage checkpoint
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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