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          • Primary Antibodies ›
          • ERK1/2 Antibodies

          Invitrogen

          Phospho-ERK1/ERK2 (Thr202, Tyr204) Monoclonal Antibody (S.812.9)

          Advanced Verification
          5 Published Figures
          7 References
          View all (81) ERK1/2 antibodies

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          Cite Phospho-ERK1/ERK2 (Thr202, Tyr204) Monoclonal Antibody (S.812.9)

          Additional Information:
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          • Antibody Testing Data (3)
          • Published Figures (5)
          • Advanced Verification (2)
          Phospho-ERK1/ERK2 (Thr202, Tyr204) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Phospho-ERK1/ERK2 (Thr202, Tyr204) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 10

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          Phospho-ERK1/ERK2 (Thr202, Tyr204) Antibody (MA5-15173) in IHC (P)

          Immunohistochemistry was performed on paraffin-embedded human colon carcinoma tissue. To expose target proteins, antigen retrieval was performed by boiling tissue sections in 10mM sodium citrate buffer, pH 6.0 for 10 minutes. Following antigen retrieval, endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 minutes, tissues were blocked in 5% normal goat serum in TBST for 1 hour at ro... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-ERK1/ERK2 (Thr202, Tyr204) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-ERK1/ERK2 (Thr202, Tyr204) Antibody in Western Blot (WB)
          Phospho-ERK1/ERK2 (Thr202, Tyr204) Antibody in ChIP Assay (ChIP)
          Phospho-ERK1/ERK2 (Thr202, Tyr204) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Phospho-ERK1/ERK2 (Thr202, Tyr204) Antibody in Immunohistochemistry (IHC)
          Phospho-ERK1/ERK2 (Thr202, Tyr204) Antibody in Western Blot (WB)
          Phospho-ERK1/ERK2 (Thr202, Tyr204) Antibody in Western Blot (WB)
          Phospho-ERK1/ERK2 (Thr202, Tyr204) Antibody in Western Blot (WB)
          Phospho-ERK1/ERK2 (Thr202, Tyr204) Antibody
          Phospho-ERK1/ERK2 (Thr202, Tyr204) Antibody
          Phospho-ERK1/ERK2 (Thr202, Tyr204) Monoclonal Antibody (S.812.9)

          Product Details

          MA5-15173

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 5 publications 5 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:400
          -

          Immunocytochemistry (ICC/IF)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          1:100
          -

          ChIP assay (ChIP)

          1-3 µL
          -
          Product Specifications

          Species Reactivity

          Fruit fly, Hamster, Human, Mink, Mouse, Non-human primate, Pig, Rat, Yeast, Zebrafish

          Published species

          Hamster, Human, Rat, Zebrafish

          Host/Isotype

          Rabbit / IgG

          Class

          Monoclonal

          Type

          Antibody

          Clone

          S.812.9

          Immunogen

          Synthetic phosphopeptide corresponding to residues surrounding pThr202/Tyr204 of human p44 MAP kinase
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          52.0 µg/mL

          Amount

          10.4 µg

          Purification

          Affinity chromatography

          Storage buffer

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

          Contains

          <0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_11009630

          Product Specific Information

          It is not recommended to aliquot this antibody.

          This antibody is not cross-reactive with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase.

          Target Information

          ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CG12559-PA; CG12559-PC; CG12559-PD; CG12559-PE; CG12559-PG; CG12559-PH; CG12559-PI; Diphospho-ERK; dp-ERK; dpERK; drosophila ERK; enhancer of seven in absentia 7; ERK; Erk MAP kinase; ERK-1; ERK-2; ERK/rolled; erk1 erk2; Erk1; cancer susceptibility protein; ERK1b; ERK1c; ERK2; Erk2; cancer susceptibility protein; ERT1; ERT2; extracellular signal-regulated kinase; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase A; extracellular signal-regulated kinase-1; extracellular signal-regulated kinase-2; extracellular signal-related kinase 1; Extracellular-regulated kinase A; extracellular-signal-regulated kinase 2; Extracellular-signal-related kinase A; Insulin-stimulated MAP2 kinase; MAP kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAP-kinase; MAPK; MAPK 1; MAPK 2; MAPK 3; Microtubule-associated protein 2 kinase; mitogen activated protein kinase; mitogen activated protein kinase 1; mitogen-activated 3; mitogen-activated protein kinase; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; Mitogen-activated protein kinase ERK-A; MNK1; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; phospho-ERK; pp42/MAP kinase; protein kinase; Protein rolled; protein tyrosine kinase ERK2; RE08694p; rl-PA; rl-PC; rl-PD; rl-PE; rl-PG; rl-PH; rl-PI; roll; sevenmaker; unnamed protein product

          View more View less

          Gene Aliases: 12559; 9030612K14Rik; AA407128; AU018647; BcDNA:RE08694; C78273; CG12559; CG18732; CT34260; CT39192; D-ERK; DERK; DERK-A; dm-dpERK; Dmel\CG12559; Dmel_CG12559; DmERK; DmERK-A; DmERKA; DmMAPK; dp-ERK; DpERK; dpERK1; dpMAPK; Dsor2; E(sina)7; EC2-1; EK2-1; ERK; ERK-1; ERK-2; ERK-A; Erk/Map kinase; ERK1; ERK1/2; ERK2; ERKA; ERT1; ERT2; Esrk1; EY2-2; fi06b09; GroupII; HS44KDAP; HUMKER1A; I79_009500; I79_018350; l(2)41Ac; l(2R)EMS45-39; MAP-k; MAPK; MAPK1; MAPK2; MAPK3; MNK1; Mtap2k; NS13; p-ERK; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; pERK; pMAPK; PRKM1; PRKM2; PRKM3; RL; rl/ERK; rl/MAPK; rll; SEM; SR2-1; Su(Raf)2B; wu:fi06b09; zERK1; zERK2

          View more View less

          UniProt ID: (Human) P28482, (Human) P27361, (Rat) P63086, (Mouse) P63085, (Mouse) Q63844, (Rat) P21708, (Fruit fly) P40417

          View more View less

          Entrez Gene ID: (Pig) 100153927, (Human) 5594, (Human) 5595, (Pig) 445013, (Chinese hamster) 100759514, (Rat) 116590, (Mouse) 26413, (Mouse) 26417, (Rat) 50689, (Chinese hamster) 100753149, (Zebrafish) 360144, (Zebrafish) 399480, (Fruit fly) 3354888

          View more View less

          Function(s)
          nucleotide binding phosphotyrosine binding DNA binding protein kinase activity protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity transferase activity phosphatase binding identical protein binding protein serine kinase activity DNA-binding transcription factor binding transcription factor binding protein kinase binding mitogen-activated protein kinase kinase kinase binding transferase activity, transferring phosphorus-containing groups scaffold protein binding non-receptor serine/threonine protein kinase
          Process(es)
          MAPK cascade regulation of transcription from RNA polymerase II promoter protein phosphorylation apoptotic process chemotaxis cellular response to DNA damage stimulus signal transduction cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway chemical synaptic transmission heart development learning or memory insulin receptor signaling pathway organ morphogenesis positive regulation of macrophage chemotaxis positive regulation of peptidyl-threonine phosphorylation neural crest cell development Schwann cell development peptidyl-threonine phosphorylation cytosine metabolic process regulation of ossification regulation of cellular pH thyroid gland development regulation of protein stability lipopolysaccharide-mediated signaling pathway positive regulation of telomere maintenance response to lipopolysaccharide regulation of stress-activated MAPK cascade mammary gland epithelial cell proliferation cellular response to amino acid starvation response to nicotine intracellular signal transduction ERBB signaling pathway ERBB2-ERBB3 signaling pathway outer ear morphogenesis myelination response to exogenous dsRNA positive regulation of cholesterol biosynthetic process negative regulation of cell differentiation insulin-like growth factor receptor signaling pathway thymus development T cell receptor signaling pathway B cell receptor signaling pathway stress-activated MAPK cascade regulation of cytoskeleton organization Bergmann glial cell differentiation long-term synaptic potentiation face development lung morphogenesis trachea formation labyrinthine layer blood vessel development cardiac neural crest cell development involved in heart development interleukin-34-mediated signaling pathway chemokine-mediated signaling pathway ERK1 and ERK2 cascade response to epidermal growth factor cellular response to tumor necrosis factor caveolin-mediated endocytosis regulation of Golgi inheritance positive regulation of macrophage proliferation positive regulation of neuroinflammatory response regulation of early endosome to late endosome transport autophagosome assembly negative regulation of T cell mediated immune response to tumor cell transcription, DNA-templated regulation of gene expression negative regulation of autophagy positive regulation of autophagy phosphorylation sensory perception of pain BMP signaling pathway positive regulation of cyclase activity cellular response to nutrient levels negative regulation of proteasomal ubiquitin-dependent protein catabolic process peptidyl-tyrosine autophosphorylation TORC1 signaling glucose homeostasis signal transduction in response to DNA damage positive regulation of transcription from RNA polymerase II promoter modulation of synaptic transmission protein stabilization negative regulation of T cell activation cartilage development positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway cellular response to mechanical stimulus negative regulation of cholesterol efflux xenophagy negative regulation of TORC1 signaling positive regulation of xenophagy MAPK import into nucleus positive regulation of cell proliferation response to toxic substance peptidyl-serine phosphorylation positive regulation of cell migration response to estrogen positive regulation of translation positive regulation of transcription, DNA-templated cellular response to organic substance cellular response to granulocyte macrophage colony-stimulating factor stimulus cell cycle regulation of sequence-specific DNA binding transcription factor activity positive regulation of protein phosphorylation protein complex assembly DNA damage induced protein phosphorylation positive regulation of histone phosphorylation positive regulation of histone acetylation negative regulation of apolipoprotein binding arachidonic acid metabolic process gastrulation eye development brain development central nervous system projection neuron axonogenesis chordate embryonic development mitotic cell cycle terminal region determination terminal branching, open tracheal system R7 cell fate commitment imaginal disc-derived wing vein specification imaginal disc-derived wing morphogenesis leg disc proximal/distal pattern formation metamorphosis torso signaling pathway determination of adult lifespan fibroblast growth factor receptor signaling pathway cellular response to starvation negative regulation of macroautophagy cellular response to reactive oxygen species lymph gland plasmatocyte differentiation lymph gland crystal cell differentiation tracheal pit formation in open tracheal system sevenless signaling pathway positive regulation of cell size positive regulation of photoreceptor cell differentiation dorsal appendage formation vascular endothelial growth factor receptor signaling pathway behavioral response to ethanol positive regulation of sequence-specific DNA binding transcription factor activity defense response to virus branching involved in open tracheal system development cellular response to arsenic-containing substance cellular response to cadmium ion positive regulation of wound healing regulation of heart morphogenesis regulation of response to drug
          It has to be done as per old AB suggested Products section.

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