Hamburger Menu Button
Thermo Fisher Scientific Logo
Iniciar sesión
¿No tiene una cuenta? Crear una cuenta
  • Productos
    • Consumibles de laboratorio
    • Equipos de laboratorio
    • Instrumentos de Laboratorio
    • Clínica y Diagnóstico
    • Cromatografía
    • Espectrometría de Masas
    • Cultivo Celular
    • Análisis Celular
    • Anticuerpos
    • Ver todas las categorías de producto
  • Aplicaciones
    • Cultivo celular y transfección
    • Citometría de flujo
    • Investigación sobre el cáncer
    • Cromatografía
    • Secuenciación
    • PCR
    • Soluciones para laboratorio
    • Diagnóstico de alergias
    • Ver todas las aplicaciones y técnicas
  • Servicios
    • Servicios Personalizados
    • Servicios de Capacitación
    • Informática para laboratorios de ámbito empresarial
    • Servicios financieros y de arrendamiento
    • Servicios 360° de CDMO y CRO
    • Servicios de CDMO
    • Servicios de CRO
    • Inspección de seguridad alimentaria Servicios
    • Ver todos los servicios
  • Ayuda y Soporte
    • Crear una nueva cuenta
    • Cómo hacer el pedido
    • Póngase en contacto con nosotros
    • Cambio de ubicación
    • Ver toda la ayuda y soporte técnico
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • A quiénes brindamos nuestros servicios
    • Industria biotecnológica
    • Sector biofarmacéutico
    • CDMO
    • Diagnósticos de laboratorio
    • Ciencias aplicadas e industriales
  • Ofertas especiales
  • Contáctenos
  • Orden Rápida
  • Documentos y certificados
Thermo Fisher Scientific Logo

Search

Buscar
Search button
          • Contáctenos
          • Orden Rápida
          • Iniciar sesión
            Iniciar sesión
            ¿No tiene una cuenta? Crear una cuenta
            • Cuenta
            • Estatus del pedido
            • Productos personalizados y proyectos​
          • Primary Antibodies ›
          • ERK1/2 Antibodies

          Invitrogen

          ERK1/ERK2 Monoclonal Antibody (3F8B3)

          Advanced Verification
          5 Published Figures
          6 References
          View all (87) ERK1/2 antibodies

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support

          Cite ERK1/ERK2 Monoclonal Antibody (3F8B3)

          Additional Information:
          {{banner.description}}
          {{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
          • Antibody Testing Data (5)
          • Published Figures (5)
          • Advanced Verification (2)
          ERK1/ERK2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          ERK1/ERK2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 12

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          ERK1/ERK2 Antibody (MA5-15605) in ICC/IF

          Immunofluorescent analysis of p44, 42 MAPK (Erk1/2) in HeLa cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a p44/42 MAPK (Erk1/2) mouse monoclonal antibody (Product # MA5-15605) at a dilution of 1:250 in blocking buffer for ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          ERK1/ERK2 Antibody in Immunocytochemistry (ICC/IF)
          ERK1/ERK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ERK1/ERK2 Antibody in Western Blot (WB)
          ERK1/ERK2 Antibody in Western Blot (WB)
          ERK1/ERK2 Antibody in Western Blot (WB)
          ERK1/ERK2 Antibody in Western Blot (WB)
          ERK1/ERK2 Antibody in Western Blot (WB)
          ERK1/ERK2 Antibody in Western Blot (WB)
          ERK1/ERK2 Antibody in Western Blot (WB)
          ERK1/ERK2 Antibody in Western Blot (WB)
          ERK1/ERK2 Antibody
          ERK1/ERK2 Antibody

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          MA5-15605

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 6 publications 6 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:300
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG2b

          Class

          Monoclonal

          Type

          Antibody

          Clone

          3F8B3

          Immunogen

          Purified recombinant fragment of human MAPK expressed in E. Coli.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10983247

          Product Specific Information

          MA5-15605 targets p44/42 MAPK (Erk1/2) in FACS, IHC, WB and IF applications and shows reactivity with Human and mouse samples.

          Target Information

          ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: ERK-2; erk1 erk2; Erk1; cancer susceptibility protein; ERK1; MAP kinase; mitogen-activated protein kinase; ERK1b; ERK1c; Erk2; cancer susceptibility protein; ERT1; extracellular signal-regulated kinase 1; extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAPK 1; MAPK 2; MAPK 3; microtubule-associated protein 2 kinase; mitogen-activated 3; mitogen-activated protein kinase 1; mitogen-activated protein kinase 2; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; pp42/MAP kinase; protein serine/threonine kinase ERK1b; protein tyrosine kinase ERK2; unnamed protein product

          View more View less

          Gene Aliases: 9030612K14Rik; ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; MAPK1; MAPK2; MNK1; Mtap2k; NS13; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3

          View more View less

          UniProt ID: (Human) P28482, (Human) P27361, (Rat) P63086, (Mouse) P63085, (Mouse) Q63844, (Rat) P21708

          View more View less

          Entrez Gene ID: (Human) 5594, (Human) 5595, (Rat) 116590, (Mouse) 26413, (Mouse) 26417, (Rat) 50689

          View more View less

          Function(s)
          phosphotyrosine binding protein kinase activity protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding identical protein binding protein serine kinase activity DNA-binding transcription factor binding double-stranded DNA binding protein kinase binding mitogen-activated protein kinase kinase kinase binding scaffold protein binding
          Process(es)
          autophagosome assembly MAPK cascade negative regulation of T cell mediated immune response to tumor cell protein phosphorylation cellular response to DNA damage stimulus signal transduction cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway insulin receptor signaling pathway response to toxic substance organ morphogenesis negative regulation of autophagy positive regulation of autophagy positive regulation of macrophage chemotaxis neural crest cell development Schwann cell development phosphorylation peptidyl-serine phosphorylation sensory perception of pain regulation of ossification BMP signaling pathway thyroid gland development positive regulation of cyclase activity lipopolysaccharide-mediated signaling pathway cellular response to nutrient levels positive regulation of telomere maintenance negative regulation of proteasomal ubiquitin-dependent protein catabolic process response to lipopolysaccharide regulation of stress-activated MAPK cascade cellular response to amino acid starvation intracellular signal transduction peptidyl-tyrosine autophosphorylation ERBB2-ERBB3 signaling pathway TORC1 signaling outer ear morphogenesis myelination signal transduction in response to DNA damage response to exogenous dsRNA positive regulation of translation positive regulation of transcription from RNA polymerase II promoter decidualization insulin-like growth factor receptor signaling pathway thymus development modulation of synaptic transmission protein stabilization negative regulation of T cell activation cartilage development stress-activated MAPK cascade regulation of intracellular pH regulation of cytoskeleton organization Bergmann glial cell differentiation face development lung morphogenesis trachea formation cardiac neural crest cell development involved in heart development interleukin-34-mediated signaling pathway protein-containing complex assembly ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway response to epidermal growth factor cellular response to mechanical stimulus cellular response to tumor necrosis factor caveolin-mediated endocytosis regulation of Golgi inheritance negative regulation of cholesterol efflux cellular response to toxic substance positive regulation of macrophage proliferation positive regulation of neuroinflammatory response negative regulation of TORC1 signaling positive regulation of xenophagy regulation of early endosome to late endosome transport arachidonic acid metabolic process regulation of transcription from RNA polymerase II promoter apoptotic process chemotaxis chemical synaptic transmission learning or memory positive regulation of peptidyl-threonine phosphorylation response to nicotine ERBB signaling pathway positive regulation of skeletal muscle tissue regeneration positive regulation of cholesterol biosynthetic process positive regulation of smoothened signaling pathway chemokine-mediated signaling pathway heart development positive regulation of cell proliferation diadenosine tetraphosphate biosynthetic process cytosine metabolic process positive regulation of cell migration response to estradiol response to testosterone mammary gland epithelial cell proliferation cellular response to platelet-derived growth factor stimulus response to cocaine positive regulation of protein import into nucleus response to hydrogen peroxide response to estrogen estrous cycle negative regulation of cell differentiation positive regulation of transcription, DNA-templated T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of cardiac muscle cell proliferation long-term synaptic potentiation labyrinthine layer blood vessel development cellular response to methionine cellular response to cAMP cellular response to epidermal growth factor stimulus cellular response to peptide hormone stimulus cellular response to prostaglandin E stimulus cellular response to lipid response to alcohol cellular response to insulin-like growth factor stimulus placenta development
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Pedidos Plus Icon Minus Icon
          • Estatus del pedido
          • Ayuda para pedidos
          • Orden Rápida
          • Supply Center
          • eProcurement
          Soporte Plus Icon Minus Icon
          • Ayuda y soporte
          • Entre en Contacto
          • Centros de asistencia técnica
          • Consultar documentos y certificados
          • Informar de un problema en la web
          Recursos Plus Icon Minus Icon
          • Centros de aprendizaje
          • Promociones
          • Eventos & Webinars
          • Medios Sociales
          Acerca de Thermo Fisher Plus Icon Minus Icon
          • Acerca de nosotros Acerca de nosotros
          • Empleo Empleo
          • Inversores Inversores
          • Noticias Noticias
          • Responsabilidad social Responsabilidad social
          • Marcas comerciales
          • Políticas y avisos
          Nuestro Portafolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Policy
          • Price & Freight Policy
          © 2006-2026 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          México flag icon
          México

          Your items have has been added!


          Host server : magellan-search-blue-76c4fffd86-nv6df:80/100.66.78.131:80.
          git-commit: bdc067a1e9e92d056899a2dda64af631988aa8a1
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.54.0-Offline