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          • Primary Antibodies ›
          • XBP1 Antibodies

          Invitrogen

          XBP1 Monoclonal Antibody (9B7E5)

          Advanced Verification
          1 Published Figure
          2 References
          View all (30) XBP1 antibodies

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          Cite XBP1 Monoclonal Antibody (9B7E5)

          Additional Information:
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          • Antibody Testing Data (4)
          • Published Figures (1)
          • Advanced Verification (1)
          XBP1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          XBP1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          XBP1 Antibody (MA5-15768) in ICC/IF

          Immunofluorescence analysis of XBP1 was performed using 70% confluent log phase MCF7 thapsigargin treated cells (2uM for 6hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with XBP1 Monoclonal Antibody (9B7E5)(Product # MA5-15768) at 5µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alex... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          XBP1 Antibody in Immunocytochemistry (ICC/IF)
          XBP1 Antibody in Immunocytochemistry (ICC/IF)
          XBP1 Antibody in Immunocytochemistry (ICC/IF)
          XBP1 Antibody in Western Blot (WB)
          XBP1 Antibody in Immunocytochemistry (ICC/IF)
          XBP1 Antibody
          XBP1 Monoclonal Antibody (9B7E5)

          Product Details

          MA5-15768

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1 µg/mL
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          9B7E5

          Immunogen

          Recombinant protein corresponding to amino acids 2 to 160 of human XBP-1.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_11154615

          Product Specific Information

          A suggested positive control is XBP-1 recombinant protein.

          Target Information

          XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Hepatocarcinogenesis-related transcription factor; HTF; OTTHUMP00000028682; Tax-responsive element-binding protein 5; tax-responsive element-binding protein 5 homolog; TREB-5; unnamed protein product; X box binding protein-1; X-box-binding protein 1; XBP-1; XBP1

          View more View less

          Gene Aliases: D11Ertd39e; HTF; TREB-5; TREB5; XBP-1; XBP1; XBP2

          View more View less

          UniProt ID: (Human) P17861, (Rat) Q9R1S4, (Mouse) O35426

          View more View less

          Entrez Gene ID: (Human) 7494, (Rat) 289754, (Mouse) 22433

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding protease binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein kinase binding estrogen receptor binding chromatin DNA binding ubiquitin protein ligase binding identical protein binding histone deacetylase binding sequence-specific DNA binding ubiquitin-like protein ligase binding protein heterodimerization activity sequence-specific double-stranded DNA binding core promoter binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding phosphatidylinositol 3-kinase regulatory subunit binding transcription regulatory region DNA binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter angiogenesis liver development positive regulation of protein phosphorylation endothelial cell proliferation epithelial cell maturation positive regulation of immunoglobulin production regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter ubiquitin-dependent protein catabolic process positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response regulation of autophagy positive regulation of autophagy negative regulation of myotube differentiation phosphatidylinositol 3-kinase signaling cell growth endoplasmic reticulum unfolded protein response exocrine pancreas development positive regulation of histone methylation regulation of protein stability protein destabilization cellular response to nutrient positive regulation of TOR signaling cellular response to insulin stimulus cellular response to oxidative stress response to endoplasmic reticulum stress cellular triglyceride homeostasis cellular response to vascular endothelial growth factor stimulus cellular response to glucose starvation response to drug glucose homeostasis cholesterol homeostasis positive regulation of transcription factor import into nucleus negative regulation of apoptotic process positive regulation of MHC class II biosynthetic process positive regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of fat cell differentiation positive regulation of transcription from RNA polymerase II promoter vascular endothelial growth factor receptor signaling pathway neuron development positive regulation of immunoglobulin secretion response to electrical stimulus fatty acid homeostasis sterol homeostasis serotonin secretion, neurotransmission adipose tissue development epithelial cell maturation involved in salivary gland development cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to antibiotic cellular response to fructose stimulus cellular response to glucose stimulus cellular response to interleukin-4 cellular response to peptide hormone stimulus cellular response to fluid shear stress cellular response to laminar fluid shear stress positive regulation of plasma cell differentiation negative regulation of endoplasmic reticulum unfolded protein response positive regulation of endoplasmic reticulum unfolded protein response positive regulation of proteasomal protein catabolic process positive regulation of protein acetylation negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of lactation response to insulin-like growth factor stimulus positive regulation of hepatocyte proliferation positive regulation of endothelial cell apoptotic process positive regulation of interleukin-6 secretion regulation of cell growth glandular epithelial cell maturation lipid metabolic process fatty acid biosynthetic process autophagy apoptotic process immune response response to unfolded protein muscle organ development positive regulation of cell proliferation protein transport cell differentiation positive regulation of cell migration negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of interleukin-6 production positive regulation of vascular wound healing IRE1-mediated unfolded protein response ATF6-mediated unfolded protein response ERAD pathway positive regulation of protein import into nucleus proteasome-mediated ubiquitin-dependent protein catabolic process protein kinase B signaling positive regulation of angiogenesis positive regulation of protein kinase B signaling negative regulation of SMAD protein import into nucleus intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress negative regulation of ERK1 and ERK2 cascade positive regulation of phospholipid biosynthetic process positive regulation of ERAD pathway positive regulation of vascular smooth muscle cell proliferation positive regulation of vascular associated smooth muscle cell migration cellular response to leukemia inhibitory factor transcription, DNA-templated transport multicellular organism development
          It has to be done as per old AB suggested Products section.

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