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Invitrogen

NFkB p50 Monoclonal Antibody (5D10D11)

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View all (7) NFkB p50 antibodies

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Product Details

MA5-15870

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
- -

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:200
View 1 publication 1 publication

Flow Cytometry (Flow)

1:50-1:200
-

ChIP assay (ChIP)

2 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5D10D11

Immunogen

Purified recombinant fragment of human NFKB1 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153885

Product Specific Information

MA5-15870 targets NFKB1 in FACS, IF, IHC, ChIP, and WB applications and shows reactivity with Human samples.

Target Information

NFkB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. NFkB is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50) is a 50KDa functional sub-unit of NF-kB and a member of Rel protein family which is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NFkB factor. Further, NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-binding. The NFkB complex is expressed in most cell types, and is primarily found in the cytoplasm in an inactive state in association with I-kappa-B. Phosphorylation of I-kappa-B releases the complex, allowing translocation to the nucleus and modification of gene expression. Phosphorylation of the p50 subunit at the Ser-337 residue has been shown to significantly increase DNA binding efficiency of the NFkB protein complex. The pathological role of NFkB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp686C01211; DNA-binding factor KBF1; EBP-1; MGC54151; NF KB; NF-kB p50; nuclear factor; nuclear factor kappa b; nuclear factor kappa-B DNA binding subunit; Nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; OTTHUMP00000219572; unnamed protein product

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Gene Aliases: CVID12; EBP-1; KBF1; NF-kappa-B1; NF-kappaB; NF-kappabeta; NF-kB; NF-kB1; NFkappaB; NFKB-p105; NFKB-p50; NFKB1

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UniProt ID: (Human) P19838

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Entrez Gene ID: (Human) 4790

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription coactivator binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription cofactor activity protein binding identical protein binding actinin binding sequence-specific DNA binding protein sequestering activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter MAPK cascade negative regulation of cytokine production immune system process regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter apoptotic process inflammatory response signal transduction I-kappaB kinase/NF-kappaB signaling JNK cascade gene expression regulation of gene expression positive regulation of macromolecule biosynthetic process negative regulation of gene expression positive regulation of macrophage derived foam cell differentiation positive regulation of cholesterol efflux positive regulation of lipid storage negative regulation of vitamin D biosynthetic process signal transduction involved in regulation of gene expression negative regulation of interleukin-12 production tumor necrosis factor-mediated signaling pathway response to muscle stretch NIK/NF-kappaB signaling negative regulation of apoptotic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of inflammatory response positive regulation of inflammatory response B cell receptor signaling pathway mammary gland involution positive regulation of transcription initiation from RNA polymerase II promoter cellular response to lipopolysaccharide cellular response to mechanical stimulus cellular response to nicotine cellular response to cytokine stimulus cellular response to interleukin-6 cellular response to tumor necrosis factor cellular response to dsRNA positive regulation of canonical Wnt signaling pathway cellular response to interleukin-17 cellular response to virus antibacterial innate immune response negative regulation of cytokine production involved in inflammatory response positive regulation of hyaluronan biosynthetic process cellular response to angiotensin positive regulation of miRNA metabolic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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