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Invitrogen
TLR4 Monoclonal Antibody (76B357.1), FITC
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FIGURE: 1 / 1
TLR4 Antibody (MA5-16219) in Flow
FACS analysis of cell surface TLR4 in human monocyte-derived dendritic cells using a TLR4/CD284 monoclonal antibody (Product # MA5-16219) at 1:200 (2.5 ug). Black and green open histograms represent unstained cells and negative control (human CD14 FITC) respectively. Blue open histogram is TLR4/CD284 monoclonal antibody (Product # MA5-16219).
Product Details
MA5-16219
Product Specifications
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
76B357.1
Immunogen
Synthetic peptide (aa 125-140) of TLR4/CD284 protein.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.78 mg/mL
Amount
78 µg
Storage conditions
4°C, store in dark
Shipping conditions
Wet ice
RRID
Product Specific Information
Suggested positive control: Ramos or ThP1.
Target Information
TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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