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Invitrogen
SOX2 Monoclonal Antibody (SP76)
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FIGURE: 1 / 9
SOX2 Antibody (MA5-16399) in ICC/IF
Immunofluorescence analysis of SOX2 was performed using 70% confluent log phase NTERA-2 cl.D1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SOX2 Monoclonal Antibody (SP76) (Product # MA5-16399) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (... View More
Product Details
MA5-16399
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
SP76
Immunogen
Synthetic peptide corresponding to N-terminus of human SOX-2 protein.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Purification
Protein A/G
Storage buffer
PBS, pH 7.6, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is prostate or lung squamous cell carcinoma.
Target Information
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); putative; sex determining region Y-box 2; SOX 2; sox-2; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY related HMG box 2; SRY-box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2; unnamed protein product
Gene Aliases: ANOP3; lcc; MCOPS3; Sox-2; SOX2; ysb
UniProt ID: (Human) P48431, (Mouse) P48432
Entrez Gene ID: (Human) 6657, (Mouse) 20674
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
miRNA binding
sequence-specific DNA binding
nitric-oxide synthase binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
chromatin binding
transcription factor binding
chromatin DNA binding
transcription regulatory region DNA binding
protein heterodimerization activity
HMG box transcription factor
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
eye development
endodermal cell fate specification
chromatin organization
regulation of transcription, DNA-templated
brain development
response to wounding
regulation of gene expression
glial cell fate commitment
pituitary gland development
adenohypophysis development
positive regulation of cell-cell adhesion
neuron differentiation
forebrain development
somatic stem cell population maintenance
tissue regeneration
positive regulation of MAPK cascade
response to ethanol
positive regulation of cell differentiation
negative regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
inner ear development
response to growth factor
cellular response to hypoxia
negative regulation of canonical Wnt signaling pathway
response to oxygen-glucose deprivation
neuronal stem cell population maintenance
negative regulation of cell cycle G1/S phase transition
regulation of myofibroblast cell apoptotic process
cell fate specification
positive regulation of neuroblast proliferation
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
cell cycle arrest
multicellular organism development
sensory perception of sound
response to organic substance
stem cell population maintenance
forebrain neuron differentiation
cerebral cortex development
negative regulation of Wnt signaling pathway
male genitalia development
positive regulation of epithelial cell differentiation
olfactory placode formation
response to retinoic acid
inner ear morphogenesis
regulation of cysteine-type endopeptidase activity involved in apoptotic process
tongue development
cell fate commitment
negative regulation of cell differentiation
positive regulation of neuron differentiation
negative regulation of osteoblast differentiation
positive regulation of Notch signaling pathway
pigment biosynthetic process
lung alveolus development
embryonic organ development
anatomical structure formation involved in morphogenesis
neuron fate commitment
diencephalon morphogenesis
stem cell differentiation
negative regulation of epithelial cell proliferation
regulation of neurogenesis
detection of mechanical stimulus involved in sensory perception of sound
detection of mechanical stimulus involved in equilibrioception
retina morphogenesis in camera-type eye
lens induction in camera-type eye
epithelial tube branching involved in lung morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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