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Invitrogen
Vimentin Monoclonal Antibody (SP20)
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FIGURE: 1 / 19
Vimentin Antibody (MA5-16409) in ICC/IF
Immunofluorescence analysis of Vimentin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Vimentin Rabbit Monoclonal Antibody (Product # MA5-16409) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate ... View More
Product Details
MA5-16409
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Non-human primate,
Sheep
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
SP20
Immunogen
Recombinant human vimentin protein.
Conjugate
Form
Liquid
Concentration
0.006 mg/mL
Amount
3 µg
Purification
Protein A
Storage buffer
PBS, pH 7.20, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Predicted to react with Rat, Hamster, Cow, and Xenopus laevis based on sequence homology.
Recommended Postive Controls - WB: U-2 OS, Hu tonsil and HeLa cell lysates. Flow: HeLa cells. ICC/IF: HAP1-VIM cells, human limbal epithelial cells. IHC-P: Human breast cancer and melanoma tissue. IHC-Fr: Colorectal cancer tissue.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; FLJ36605; Hel113; RP11-124N14.1; unnamed protein product; VIM; VIME; Vimentin
Gene Aliases: VIM
UniProt ID: (Human) P08670, (Mouse) P20152
Entrez Gene ID: (Human) 7431, (Mouse) 22352
RNA binding
double-stranded RNA binding
structural constituent of cytoskeleton
structural constituent of eye lens
protein binding
protein domain specific binding
identical protein binding
binding, bridging
scaffold protein binding
keratin filament binding
glycoprotein binding
structural molecule activity
protein C-terminus binding
kinase binding
protein kinase binding
protein phosphatase 2A binding
positive regulation of gene expression
negative regulation of neuron projection development
astrocyte development
intermediate filament-based process
intermediate filament organization
regulation of axonogenesis
Bergmann glial cell differentiation
positive regulation of glial cell proliferation
SMAD protein signal transduction
lens fiber cell development
regulation of Schwann cell migration
neuron projection development
positive regulation of collagen biosynthetic process
regulation of mRNA stability
cellular response to lipopolysaccharide
cellular response to muramyl dipeptide
cellular response to interferon-gamma
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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