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Invitrogen
Nidogen G3 Domain Monoclonal Antibody (JF2)
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Product Details
MA5-17020
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
JF2
Immunogen
Recombinant fragment corresponding to the G3 region of mouse nidogen produced in Fisher rat plus YB2/0 cells
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
2M tris, 0.5M acetic acid
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes native and denatured mouse and human nidogen, and blocks binding of nidogen to laminin.
It has been successfully used in ELISA and Western blot applications.
The immunogen is a recombinant fragment corresponding to the G3 region of mouse nidogen produced in Fisher rat plus YB2/0 cells.
Target Information
Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Although the precise composition and structure of basal laminae varies from tissue to tissue, as well as within the same tissue at different developmental stages, most mature basal laminae contain type IV collagen, laminins, heparan sulphate proteoglycans and entactin (or nidogen). Entactin is a sulfated glycoprotein that is widely distributed in basement membranes. It is tightly associated with laminin and also binds to collagen IV. Entactin probably has a role in cell-extracellular matrix interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: enactin; Ent1; entactin; entactin 1; epididymis secretory sperm binding protein; NID 1; NID-1; Nidogen (enactin); Nidogen precursor (Entactin); unnamed protein product
Gene Aliases: A630025O17; entactin; entactin-1; NID; nidogen-1
UniProt ID: (Human) P14543, (Mouse) P10493
Entrez Gene ID: (Human) 4811, (Mouse) 18073
cell-matrix adhesion
positive regulation of cell adhesion
positive regulation of muscle cell differentiation
basement membrane organization
regulation of basement membrane organization
positive regulation of integrin-mediated signaling pathway
positive regulation of cell-substrate adhesion
extracellular matrix organization
glomerular basement membrane development
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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