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Invitrogen
RUNX3 Monoclonal Antibody (2B3)
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FIGURE: 1 / 19
RUNX3 Antibody (MA5-17169) in ICC/IF
Immunofluorescence analysis of RUNX3 was performed using 70% confluent log phase SW480 and Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RUNX3 Monoclonal Antibody (2B3) (Product # MA5-17169) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Produ... View More
Product Details
MA5-17169
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
2B3
Immunogen
Purified recombinant fragment of human RUNX3 (amino acids:186-252) expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-17169 targets RUNX3 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Mouse samples.
The MA5-17169 immunogen is purified recombinant fragment of human RUNX3 (amino acids:186-252) expressed in E. Coli.
MA5-17169 detects RUNX3 which has a predicted molecular weight of approximately 44.4kDa.
Target Information
This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Acute myeloid leukemia 2 protein; acute myeloid leukemia gene 2; CBF-alpha 3; CBF-alpha-3; core binding factor alpha 3; Core-binding factor subunit alpha-3; core-binding factor, runt domain, alpha subunit 3; Oncogene AML-2; PEA2 alpha C; PEA2-alpha C; PEBP2 alpha C; PEBP2-alpha C; Polyomavirus enhancer-binding protein 2 alpha C subunit; runt domain, alpha subunit 3; runt related transcription factor 3; Runt-related transcription factor 3; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit; transcription factor AML2; transcription factor AML2/CBFA3; unnamed protein product
Gene Aliases: AML2; CBFA3; PEBP2A3; PEBP2aC; RUNX3
UniProt ID: (Human) Q13761, (Mouse) Q64131
Entrez Gene ID: (Human) 864, (Mouse) 12399
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription corepressor binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
ATP binding
sequence-specific double-stranded DNA binding
Runt transcription factor
negative regulation of transcription from RNA polymerase II promoter
ossification
chondrocyte differentiation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein phosphorylation
hemopoiesis
neuron differentiation
negative regulation of CD4-positive, alpha-beta T cell differentiation
positive regulation of CD8-positive, alpha-beta T cell differentiation
regulation of cell differentiation
negative regulation of cell cycle
positive regulation of transcription, DNA-templated
peripheral nervous system neuron development
negative regulation of epithelial cell proliferation
response to transforming growth factor beta
transcription, DNA-templated
axon guidance
hair follicle morphogenesis
neuron projection development
interferon-gamma production
mRNA transcription from RNA polymerase II promoter
cell maturation
positive regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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