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Invitrogen
TUBA8 Monoclonal Antibody (2D6)
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FIGURE: 1 / 8
TUBA8 Antibody (MA5-17193) in ICC/IF
Immunofluorescence analysis of TUBA8 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TUBA8 Monoclonal Antibody (2D6) (Product # MA5-17193) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-17193
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Algae,
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
2D6
Immunogen
Purified recombinant fragment of human TUBA8 expressed in E. Coli.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-17193 targets TUBA8 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Rat samples.
The MA5-17193 immunogen is purified recombinant fragment of human TUBA8 expressed in E. Coli.
MA5-17193 detects TUBA8 which has a predicted molecular weight of approximately 50kDa.
Target Information
This gene encodes a member of the alpha tubulin protein family. Alpha tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in this gene are associated with polymicrogyria and optic nerve hypoplasia. Alternate splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha tubulin 8; alpha-tubulin 8; cytoskeletal protein; TUBA8; tubulin alpha 8 trucated form; tubulin alpha chain-like 2; unnamed protein product
Gene Aliases: CDCBM8; MACTHC2; TUBAL2
UniProt ID: (Human) Q9NY65, (Mouse) Q9JJZ2, (Rat) Q6AY56
Entrez Gene ID: (Human) 51807, (Mouse) 53857, (Rat) 500377
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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