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Invitrogen
H3K27me2 Monoclonal Antibody
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FIGURE: 1 / 3
H3K27me2 Antibody (MA5-23511) in WB
Western blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), MDA-MB-231 (Lane 3), HCT 116 (Lane 4) and U-2 OS (Lane 5). The blot was probed with Di-Tri-Methyl-Histone H3 (Lys27) (Product # MA5-23511, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to Di-Tri-Methyl-Histone H3 (Lys27) was observed across the cell lines tested.
Product Details
MA5-23511
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide corresponding to human histone H3, di- or trimethylated at lysine 27 (H3K27me2/3).
Conjugate
Form
Liquid
Concentration
0.9 mg/mL
Amount
50 µg
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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