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Invitrogen
ME1 Monoclonal Antibody (3I6)
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FIGURE: 1 / 6
ME1 Antibody (MA5-23524) in IHC (P)
ME1 Monoclonal Antibody (3I6) detects ME1 protein at cytoplasm in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. ME1 Monoclonal Antibody (3I6) (Product # MA5-23524) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-23524
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
3I6
Immunogen
Recombinant protein encompassing a sequence within the center region of human ME1. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, and HeLa cells.
Predicted reactivity: Mouse (91%), Rat (91%), Chicken (80%), Sheep (92%), Bovine (91%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ME1 encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)); malic enzyme; malic enzyme 1, NADP(+)-dependent, cytosolic; malic enzyme 1, soluble; Malic enzyme, cytoplasmic; malic enzyme, supernatant; NADP-ME; pyruvic-malic carboxylase; unnamed protein product
Gene Aliases: D9Ertd267e; HUMNDME; Mdh-1; MES; Mod-1; Mod1
UniProt ID: (Human) P48163, (Mouse) P06801
Entrez Gene ID: (Human) 4199, (Mouse) 17436
magnesium ion binding
malic enzyme activity
malate dehydrogenase (decarboxylating) (NADP+) activity
protein binding
oxaloacetate decarboxylase activity
electron carrier activity
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
manganese ion binding
identical protein binding
ADP binding
NADP binding
NAD binding
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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