Search
Invitrogen
ALPL Recombinant Rabbit Monoclonal Antibody (3G2)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
Product Details
MA5-24845
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
3G2
Immunogen
KLH conjugated synthetic peptide derived from human Alkaline Phosphatase: 18-50
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.03% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Purity is > 95% by SDS-PAGE.
Target Information
Alkaline phosphatase is a homodimeric protein enzyme (86 kDa), containing two zinc atoms crucial to its catalytic function per monomer, and it is optimally active at alkaline pH environments. The enzyme is found in prokaryotes and eukaryotes, with the same general function but in different structural forms suitable to organism's environment. ALP plays a role in liver metabolism and development within the skeleton. In bacteria, ALP acts as a source of inorganic phosphate. In yeast, ALP can be a useful protein for studying membrane protein sorting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Alkaline phosphatase 2; alkaline phosphatase 2, liver; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; liver/bone/kidney-type alkaline phosphatase; MGC161443; MGC167935; Phosphoamidase; Phosphocreatine phosphatase; tissue non-specific alkaline phosphatase; tissue-nonspecific alkaline phosphatase; tissue-nonspecific ALP; Tissue-nonspecific ALP alkaline phosphatase; TNSALP; unnamed protein product
Gene Aliases: Akp-2; Akp2; ALPL; AP-TNAP; APTNAP; HOPS; HPPA; HPPC; HPPI; HPPO; PHOA; TNALP; TNAP; TNS-ALP; TNSALP
UniProt ID: (Human) P05186, (Rat) P08289, (Mouse) P09242
Entrez Gene ID: (Human) 249, (Rat) 25586, (Mouse) 11647
alkaline phosphatase activity
inorganic diphosphatase activity
calcium ion binding
pyrophosphatase activity
hydrolase activity
phosphatase activity
ATPase activity
pyridoxal phosphatase activity
adenosine-diphosphatase activity
metal ion binding
phosphoamidase activity
phosphoethanolamine phosphatase activity
catalytic activity
protein binding
phosphatase
skeletal system development
osteoblast differentiation
endochondral ossification
developmental process involved in reproduction
cellular homeostasis
bone mineralization
biomineral tissue development
response to lipopolysaccharide
response to insulin
response to vitamin D
response to vitamin B6
response to macrophage colony-stimulating factor
pyridoxal phosphate metabolic process
response to antibiotic
response to glucocorticoid
phosphate ion homeostasis
calcium ion homeostasis
cementum mineralization
positive regulation of cold-induced thermogenesis
futile creatine cycle
inhibition of non-skeletal tissue mineralization
response to sodium phosphate
dephosphorylation
cellular response to organic cyclic compound
metabolic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support