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FIGURE: 1 / 16
Profilin 1 Antibody (MA5-25120) in ICC/IF
Immunofluorescence analysis of Profilin 1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Profilin 1 Monoclonal Antibody (OTI2D2) (Product # MA5-25120) at 1:100 dilution in 0.1% BSA, incubated at 4 ... View More
Product Details
MA5-25120
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Non-human primate,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI2D2
Immunogen
Full length human recombinant protein of PFN1 produced in HEK293T cell
Conjugate
Form
Liquid
Concentration
1.00 mg/mL
Amount
100 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role in both signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Profilin I appears to be highly expressed in most tissues except for skeletal muscle. Profilin II is predominantly expressed in brain and at lower levels in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Epididymis tissue protein Li 184a; PFN1; profilin; Profilin I; Profilin-1; unnamed protein product
Gene Aliases: ALS18; PFN1
UniProt ID: (Human) P07737, (Rat) P62963
Entrez Gene ID: (Dog) 607397, (Human) 5216, (Rat) 64303
adenyl-nucleotide exchange factor activity
phosphotyrosine binding
RNA binding
actin binding
actin monomer binding
protein binding
phosphatidylinositol-4,5-bisphosphate binding
small GTPase binding
cadherin binding
proline-rich region binding
receptor binding
Rho GTPase binding
poly(A) RNA binding
actin or actin-binding cytoskeletal protein
cytoskeletal protein
non-motor actin binding protein
neural tube closure
regulation of transcription from RNA polymerase II promoter
positive regulation of epithelial cell migration
actin cytoskeleton organization
regulation of actin filament polymerization
positive regulation of actin filament polymerization
negative regulation of actin filament bundle assembly
positive regulation of actin filament bundle assembly
modulation of synaptic transmission
protein stabilization
negative regulation of stress fiber assembly
synapse maturation
modification of postsynaptic actin cytoskeleton
regulation of actin filament organization
positive regulation of ruffle assembly
actin polymerization or depolymerization
response to organic substance
negative regulation of actin filament polymerization
positive regulation of ATPase activity
positive regulation of transcription from RNA polymerase II promoter
positive regulation of viral transcription
positive regulation of DNA metabolic process
positive regulation of stress fiber assembly
cellular response to growth factor stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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