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Invitrogen
CDCA8 Monoclonal Antibody (OTI7G7)
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FIGURE: 1 / 4
CDCA8 Antibody (MA5-26896) in IHC (P)
Immunohistochemistry was performed on paraffin-embedded human lymphoma tissue. To expose target proteins, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min. Following antigen retrieval, tissues were probed with a CDCA8 monoclonal antibody (Product # MA5-26896) at a dilution of 1:150.
Product Details
MA5-26896
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI7G7
Immunogen
Full length human recombinant protein of CDCA8 produced in E.coli
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
CDCA8 is a component of a chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The chromosomal passenger complex, which includes Survivin, CDCA8, INCENP and Aurora-B, is known to play crucial roles during mitosis and cell division. It was found that CDCA8 interacting with Aurora-B, INCENP and Survivin, increases during G2/M phase and then reduces after exit from M phase. CDCA8 is cell cycle regulated, down-regulated in response to p53/Rb-signaling, and up-regulated in many types of cancerous tissues. In Drosophila cells, inactivation of CDCA8 results in polyploidy, delayed mitosis and abnormal tissue development, indicating its critical role for cell proliferation. Recent studies show that CDCA8 is essential for cell proliferation during early embryonic development, and its early embryonic lethality cannot be rescued by the loss of p53. Its aberrant expression is linked to a poor prognosis for gastric cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Borealin; Cell division cycle-associated protein 8; Dasra B; Dasra-B; hDasra-B; HPESCRG3; Pluripotent embryonic stem cell-related gene 3 protein; unnamed protein product
Gene Aliases: BOR; BOREALIN; CDCA8; DasraB; MESRGP; PESCRG3
UniProt ID: (Human) Q53HL2
Entrez Gene ID: (Human) 55143
mitotic sister chromatid segregation
mitotic cell cycle
mitotic cytokinesis
mitotic metaphase plate congression
mitotic spindle midzone assembly
chromosome organization
cell division
positive regulation of mitotic cell cycle spindle assembly checkpoint
mitotic spindle assembly
positive regulation of mitotic sister chromatid separation
positive regulation of attachment of mitotic spindle microtubules to kinetochore
positive regulation of mitotic cytokinesis
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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