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Invitrogen
Pellino 1 Monoclonal Antibody (OTI10D1)
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FIGURE: 1 / 2
Pellino 1 Antibody (MA5-26914) in IHC (P)
Immunohistochemistry was performed on paraffin-embedded human lymph node tissue. To expose target proteins, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min. Following antigen retrieval, tissues were probed with a PELI1 monoclonal antibody (Product # MA5-26914) at a dilution of 1:150.
Product Details
MA5-26914
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI10D1
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-245 of PELI1 produced in E.coli
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
The Pellino proteins are a highly homologous family of E3 ubiquitin ligases that act as upstream mediators in Toll-like receptor (TLR) pathways that lead to activation of MAP kinases and transcription factors. Pellino 1 is required for interleukin-1-mediated signaling through its interaction with the IRAK4-IRAK-TRAF6 complex, ultimately resulting in the activation of NF-Kappa-B. Like other members of the Pellino family, Pellino 1 is an E3 ubiquitin ligase, able to catalyze the polyubiquitination of IRAK1. It is activated via phosphorylation by either IRAK1 and IRAK4 or the IKK-related kinases IKK-epsilon; and TBK1. In addition to phosphorylation, Pellino 1 activity is also modulated via ubiquitination and sumoylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase pellino homolog 1; pellino homolog 1; Pellino-1; Pellino-related intracellular-signaling molecule; pellino1; protein pellino homolog 1; RING-type E3 ubiquitin transferase pellino homolog 1; unnamed protein product
Gene Aliases: PELI1; PRISM
UniProt ID: (Human) Q96FA3
Entrez Gene ID: (Human) 57162
protein polyubiquitination
double-strand break repair via homologous recombination
positive regulation of cytokine production
DNA repair
cellular response to DNA damage stimulus
regulation of Toll signaling pathway
protein ubiquitination
positive regulation of B cell proliferation
positive regulation of protein ubiquitination
response to lipopolysaccharide
positive regulation of toll-like receptor 3 signaling pathway
positive regulation of toll-like receptor 4 signaling pathway
TORC1 signaling
T cell proliferation
T cell activation
negative regulation of T cell proliferation
positive regulation of I-kappaB kinase/NF-kappaB signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
response to dsRNA
negative regulation of T cell activation
positive regulation of B cell activation
regulation of necroptotic process
negative regulation of necroptotic process
protein K63-linked ubiquitination
protein K48-linked ubiquitination
negative regulation of TORC1 signaling
positive regulation of TORC1 signaling
positive regulation of double-strand break repair via homologous recombination
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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