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Invitrogen
ATP5I Monoclonal Antibody (OTI1E6)
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FIGURE: 1 / 4
ATP5I Antibody (MA5-27530) in IHC (P)
Immunohistochemistry was performed on paraffin-embedded human appendix tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a ATP5I monoclonal antibody (Product # MA5-27530) at a dilution of 1:500.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-27530
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1E6
Immunogen
Full length human recombinant protein of ATP5I produced in E.coli
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. The two complexes are linked by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled, via a rotary mechanism of the central stalk subunits, with proton translocation across the membrane. ATP5I, also known as mitochondrial ATP synthase subunit E or ATP5K, is a 69 amino acid protein member of the ATPase E subunit family. Localized to the inner membrane of the mitochondria, ATP5I is a part of the F0 complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP synthase e chain, mitochondrial; ATP synthase subunit e, mitochondrial; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E; ATP synthase, H+ transporting, mitochondrial Fo complex subunit E; ATPase subunit e; F1F0-ATP synthase, murine e subunit
Gene Aliases: ATP5I; ATP5K
UniProt ID: (Human) P56385
Entrez Gene ID: (Human) 521
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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