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Invitrogen
FKBP12 Monoclonal Antibody (7D10C4H3)
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FIGURE: 1 / 5
FKBP12 Antibody (MA5-29277) in ICC/IF
Immunofluorescence analysis of Peptidyl-prolyl cis-trans isomerase FKBP1A was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with FKBP12 Monoclonal Antibody (7D10C4H3) (Product # MA5-29277) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondar... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-29277
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
7D10C4H3
Immunogen
Recombinant Human FKBP1A protein (Met1-Glu108)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human FKBP1A/FKBP12.
Target Information
FKBP12 is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 12 kDa FK506-binding protein; 12 kDa FKBP; calstabin-1; EC 5.2.1.8; FK506 binding protein 1A, 12kDa; FK506 binding protein12; FK506-binding protein (FKBP); FK506-binding protein 1; FK506-binding protein 12; FK506-binding protein 1A; FK506-binding protein, T-cell, 12-kD; FKBP12-Exip3; FKBP1A; immunophilin FKBP12; PPIase FKBP1A; protein kinase C inhibitor 2; rotamase
Gene Aliases: FKBP-12; FKBP-1A; FKBP1; FKBP12; PKC12; PKCI2; PPIASE
UniProt ID: (Human) P62942
Entrez Gene ID: (Human) 2280
peptidyl-prolyl cis-trans isomerase activity
transforming growth factor beta receptor binding
calcium channel regulator activity
protein binding
macrolide binding
FK506 binding
receptor inhibitor activity
type I transforming growth factor beta receptor binding
ion channel binding
I-SMAD binding
activin receptor binding
heart morphogenesis
protein folding
'de novo' protein folding
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion
negative regulation of transforming growth factor beta receptor signaling pathway
regulation of protein localization
negative regulation of activin receptor signaling pathway
protein refolding
T cell activation
positive regulation of I-kappaB kinase/NF-kappaB signaling
regulation of immune response
protein maturation
ventricular cardiac muscle tissue morphogenesis
heart trabecula formation
fibril organization
regulation of amyloid precursor protein catabolic process
amyloid fibril formation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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