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Invitrogen
ATP6V1A Monoclonal Antibody (GT1561)
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FIGURE: 1 / 5
ATP6V1A Antibody (MA5-31554) in WB
Western blot was performed using Anti-ATP6V1A Monoclonal Antibody (GT1561) (Product # MA5-31554) and a 70 kDa band corresponding to V-type proton ATPase catalytic subunit A was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), SiHa (Lane 3), THP-1 (Lane 4), Reh (Lane 5), Mouse Brain (Lane 6), Rat Brain (Lane 7), Mouse Skeletal Muscle (Lane 8), Rat Skeletal Muscle (Lane 9), Mouse Heart (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BO... View More
Product Details
MA5-31554
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
GT1561
Immunogen
Recombinant protein encompassing a sequence within the center region of human ATP6V1A. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.8 mg/mL
Amount
80 µg
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (96%), Rat (95%), Xenopus laevis (90%), Pig (96%), Chicken (82%), Rhesus Monkey (99%), Bovine (98%).
Positive Control: 293T, HepG2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATP6V1A encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 70-kDa subunit; ATPase, H+ transporting, lysosomal (vacuolar proton pump), alpha 70 kDa, isoform 2; ATPase, H+ transporting, lysosomal 70kD, V1 subunit A, isoform 1; ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A; ATPase, H+ transporting, lysosomal, subunit A1; ATPase, H+ transporting, V1 subunit A, isoform 1; ATPase, H+ transporting, V1 subunit A1; H(+)-transporting two-sector ATPase, subunit A; H+-transporting ATPase chain A, vacuolar (VA68 type); lysosomal 70kDa; unnamed protein product; V-ATPase 69 kDa subunit; V-ATPase 69 kDa subunit 1; V-ATPase A subunit 1; V-ATPase subunit A; V-type proton ATPase (V-ATPase) catalytic subunit A; V-type proton ATPase catalytic subunit A; Vacuolar ATPase isoform VA68; vacuolar proton pump alpha subunit 1; Vacuolar proton pump subunit alpha; vacuolar-type H(+)-ATPase
Gene Aliases: AI647066; ARCL2D; ATP6A1; Atp6a2; ATP6V1A; ATP6V1A1; DEE93; HO68; IECEE3; VA68; Vma1; VPP2
UniProt ID: (Human) P38606, (Mouse) P50516
Entrez Gene ID: (Human) 523, (Mouse) 11964, (Rat) 685232
ion transport
cellular iron ion homeostasis
vacuolar acidification
lysosomal lumen acidification
regulation of macroautophagy
cellular response to increased oxygen levels
homeostatic process
ATP metabolic process
endosomal lumen acidification
intracellular pH reduction
Golgi lumen acidification
synaptic vesicle lumen acidification
hydrogen ion transmembrane transport
transport
ATP hydrolysis coupled proton transport
proton transport
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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