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Invitrogen
Phospho-PKR (Thr446) Recombinant Rabbit Monoclonal Antibody (SY230)
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FIGURE: 1 / 4
Phospho-PKR (Thr446) Antibody (MA5-32086) in ICC/IF
Immunocytochemistry analysis of Phospho-PKR (Thr446) with SiHa cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37°C, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum (30 min, room temp), incubated with recombinant monoclonal Phospho-PKR (Thr446) (Product # MA5-32086) at a dilution of 1:50 (in 2% negative goat serum overnight at 4°C), ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-32086
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SY230
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Thr446 of Human PKR aa 421-470.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
EIF2AK2 (PKR) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses. EIF2AK2 plays a key role in antiviral defense mediated through its direct activation by double-stranded RNA produced by viral infection. PKR plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth & differentiation. PKR induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway. Upon viral infection, it functions as a dual protein, sequentially activating both cell survival & cell death pathways using kinase independent and dependent strategies. PRKR regulates multiple pathways which include NF-kappaB activation, p53, p38, & PDGF signaling pathway. PKR has been implicated in tumor suppression & malignancy. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: double stranded RNA activated protein kinase; EC 2.7.11.1; eIF-2A protein kinase 2; eIF2a Kinase; interferon-inducible elF2alpha kinase; MGC126524; OTTHUMP00000201320; P1/eIF-2A protein kinase; p68 kinase; PRKR; protein kinase R; protein kinase RNA-regulated; protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; tyrosine-protein kinase EIF2AK2; unnamed protein product
Gene Aliases: PKR; PPP1R83; PRKR
UniProt ID: (Human) P19525
Entrez Gene ID: (Human) 5610
RNA binding
double-stranded RNA binding
protein kinase activity
protein serine/threonine kinase activity
eukaryotic translation initiation factor 2alpha kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
protein binding
ATP binding
kinase activity
protein phosphatase regulator activity
identical protein binding
protein serine kinase activity
positive regulation of cytokine production
regulation of translational initiation
negative regulation of cell proliferation
response to virus
negative regulation of translation
positive regulation of chemokine production
positive regulation of stress-activated MAPK cascade
negative regulation of osteoblast proliferation
cellular response to amino acid starvation
response to interferon-alpha
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAPK cascade
negative regulation of viral genome replication
defense response to virus
antiviral innate immune response
regulation of NLRP3 inflammasome complex assembly
positive regulation of NIK/NF-kappaB signaling
regulation of hematopoietic progenitor cell differentiation
regulation of hematopoietic stem cell proliferation
regulation of hematopoietic stem cell differentiation
negative regulation of cytoplasmic translation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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